Nippon Bambanker hRM - Model BBH04 -Serum-Free Cryopreservation Media for ES and iPS Cells

A new hope for the cryopreservation of ES and iPS cells

Primate embryonic stem cells and induced-pluripotent stem cells are extremely sensitive to cryopreservation which presents many difficulties when compared to murine or other cells. At present, the vitrification method is considered adequate for primate ES/iPS cells although the slow-freezing method using DMSO has been popular for a wide variety of cell lines. Vitrification is the rapid cooling of the freezing media to a glass-like crystalline state. The vitrification method requires impeccable timing, a high level of skill, and still can yield poor results. In addition, this method shows sensitivity to dry ice transportation. To address these problems, the new freezing medium Bambanker^TM HRM has been developed.

What makes Bambanker^TM HRM so special?

The Cell freezing media Bambanker^TM HRM contains no bovine serum albumin, which can sometimes lead to cell differentiation. Furthermore, there are no animal-derived material which improve storage and survivability of primate ES/iPS cells while greatly simplifying the protocol.

Cell freezing without Serum!

All Bambanker^TM products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of Bambanker^TM is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.

Flexible

There a four different versions of Bambanker™.

• All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
• They also do not contain antibiotic or antifungal ingredients.

The four different types of Bambanker™ are recommended depending on your specific needs:

Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 100 mL)
Please note that the 120 mL bottle size (BB01) is no longer available and has been replaced by the new 100 mL bottle size (BB05).

Bambanker™ Direct (for hybridoma cells, package unit: 20 mL)

Bambanker™ DMSO Free (for high sensitivity cells, package unit: 20 mL)

Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 100 mL)
Please note that the 120 mL bottle size (BBH03) is no longer available and has been replaced by the new 100 mL bottle size (BBH04).

Customer Product Feedback

Comparison of the cryopreservation efficiency for human iPS cells

Human iPSC 201B7 cells were cryopreserved in Bambanker^TM HRM, in a conventional vitrification medium or in a 10% DMSO/culture medium. After 3 days, the cells were thawed, according to protocol, and then plated. In case of 10% DMSO containing medium only small colonies could be recovered what suggests a small survival rate, while with Bambanker^TM HRM it was possible to recover large colonies with almost the same size as achieved with vitrification freezing preservation solution. These results indicate that the same storage efficiency can be achieved with Bambanker^TM HRM as with the vitrification preservation solution with the additional advantage of an easier handling.

Comparison of cryopreservation efficiency for the common marmoset fibroblast cells intended for the iPS cell induction

Storage efficiency of Bambanker^TM and two other commercial preservative solutions (supplier T, supplier S) for fibroblasts from common Marmoset was compared. Fibroblast were cultured in a 6 cm petri dish until a confluency of 90-100%. The cells were frozen for two months at -80°C.

24 and 48 h after thawing photomicrographs were taken for every preservation solution. The percentage of dead cells was determined. The highest survival rate was achieved with Bambanker^TM HRM, followed by Supplier Ts storage solution. Supplier Ss storage solution achieved the lowest survival rate. Only the cells which were stored with Bambanker^TM HRM showed a sufficient number so that they could be directly used for iPS cell induction.