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Stemgent - Model 05-0014 - Oct4 mRNA, Human Stem Cell Reagent
Encodes the human Oct4 protein. Stemgent Oct4 mRNA encodes the Oct4 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA. Repeated transfection of Oct4 mRNA in combination with mRNAs encoding Klf4, Sox2, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.
Product Name: Oct4 mRNA, Human
Catalog Number: 05-0014
Size: 20 µg
Formulation: 100 ng/µl in RNase-free TE buffer, pH 7.0
Storage and Stability: Store at or below −70 °C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.
Quality Control: Proper protein expression and localization have been confirmed by immunocytochemistry.
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