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- IHC-plus - Model LS-B2526 - Polyclonal ...
IHC-plus - Model LS-B2526 - Polyclonal Rabbit anti-Human Insulin Antibody (IHC, IF)
Insulin antibody LS-B2526 is an unconjugated rabbit polyclonal antibody to Insulin from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
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antigen retrieval
secondary antibody
polyclonal antibody
Formalin-Fixed Paraffin-Embedded Tissue
primary antibody
immunohistochemistry
- Antibody: Insulin Rabbit anti-Human Polyclonal Antibody
- Application: IHC, IHC-P, ICC, IF, Flo
- Reactivity: Human, Mouse, Rat
- Format: Unconjugated, Unmodified
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide (KLH-coupled) derived from the sequence of human insulin.
Specificity
Recognizes endogenous levels of total human insulin protein.
- IHC
- IHC - Paraffin (1:100)
- ICC
- Immunofluorescence
- Flow Cytometry
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2526 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2526 was determined to be 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.