RCM, the advanced re-scan confocal microscopy system by Confocal NL, represents a significant step forward in imaging technology. It incorporates a digital scanner technology that standardizes re-scanning and achieves a speed of 2 frames per second at a resolution of 512x512 pixels. The device is optimized for super-resolution imaging and is equipped with high numerical aperture objectives at low magnification levels like 40x 1.4. This design allows for an enlarged field of view, brighter imagery, and reduced laser power. Notably, RCM operates efficiently even at an excitation power of just 10 nano-watts, a testament to its sensitivity. It integrates well with modern sCMOS cameras, enabling effective resolution sampling of low magnification objectives and offering compatibility for deep 3D imaging, including applications like single-molecule detection and large sample imaging. RCM's design prioritizes sharpness and high signal-to-noise ratio, even in low-fluorescence samples, minimizing phototoxic and photobleaching effects during live-cell imaging, thus enhancing the study of subcellular structures.
RCM is our second-generation RCM with digital scanner technology. It makes re-scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM has demonstrated imaging at 10 nano-watt excitation power!
The improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.
Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 3D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).
In combination with silicon objectives, RCM allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.
Using RCM with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.
Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.
Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.
Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.
RCM
RCM2.5
