Chema Diagnostica -Model Alkaline Phosphatase FL IFCC -Reagent for Quantitative In Vitro Determination
Reagent for quantitative in vitro determination of alkaline phosphatase in biological fluids.
The enzyme alkaline phosphatase (EC 3.1.3.1, orthophosphoric-monoester phosphohydrolase) hydrolizes the 4-NPP to release 4-nitrophenol, under alkaline conditions. The 4-nitrophenol formed is detected spectrophotometrically at 405 nm to give a measurement of alkaline phosphatase activity in the sample. The present method has been made according to IFCC.
The alkaline phosphatase is present in practically all tissues of the body, and it occurs at particularly high levels in intestinal epithelium, kidney tubules, bone, liver, and placenta. Although the precise metabolic function of the enzyme is not yet understood, it appears that the enzyme is associated with lipid transport in the intestine and with the calcification process in bone.