Model Sub-Type 1B -Recombinant Core protein, HCV
Recombinant Hepatitis C virus Core protein is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 115 amino acids with a molecular mass of 13100 Da. The product represents amino acids 1 to 115 of the HCV polyprotein, covering the N-terminal region (“domain I”) of the mature Core protein. It lacks the C-terminal signal peptide, the transmembrane region and the ER anchoring sequence (“domain II”) of the native protein.
- p21, nucleocapsid protein
- Source: Genetically engineered Escherichia coli
- Stabilizers: None
- Preservatives: None
- Diluent: Antigen specific solution, contains either 8M Urea + 2mM DTE or 0.1% SDS. Antigen lots in PBS / 0.1% SDS are prepared on request only.
- Purification: Ion Exchange Chromatography
- Purity: 98%, analysed by SDS-PAGE (reducing and non-reducing) followed by Coomassie-Blue staining and immunoprobing of Western blotted proteins with anti-E.coli serum (DAKO)
- Endotoxin: Not determined
- Protein concentration: Determined spectrophotometrically at 280nm (ext. coeff. 32410) and densitometrically by Advanced Image Data Analyzer software (Raytest) in relation to BSA standards
The integrity of the plasmid is controlled by restriction analysis and DNA sequencing. The expression product is characterized by N-terminal protein sequencing, SDS-PAGE (app. m.w.) and immunodetection of the Western blotted protein. In addition, correct reactivities compared to previous antigen lots with defined human and animal sera (negative, low, medium and high positive) are mandatory.
Storage and Shelf Life
At -80°C 10 years from the date of production
Avoid repeated freeze-thaw cycles