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HuabioModel GARS -SC0651 ET1610-67 - Recombinant Rabbit Monoclonal Antibody

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Western blot analysis of GARS on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

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Advantages of Using rAbs
Increased Reproducibility

Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability. Since rAbs are defined by the sequences that encode them, they are more reliable and provide more reproducible results than mAbs. By adjusting experimental conditions, researchers can easily favor the isolation of antibodies against antigens.

Ease of Scalability and Continuous Supply

In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Moreover, since the recombinant antibody sequence is known, continuity of supply is assured. This means antibody expression can be carried out at any scale with guaranteed long-term supply giving you added peace of mind and continuity for projects of all sizes, making recombinant antibodies a great solution for long-term studies.

Animal-Free Tech

Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.

  • Product Type: Recombinant Rabbit monoclonal primary
  • Product Name: GARS Recombinant Rabbit Monoclonal Antibody [SC0651] (ET1610-67)
  • Immunogen: Synthetic peptide within human gars aa 698-739 / 739.
  • Host: Rabbit
  • Positive Control: Raji cell lysates, mouse colon tissue, mouse heart tissue, human pancreas tissue.
  • Conjugation: Unconjugated
  • Clonality: Monoclonal
  • Clone Number: SC0651
  • WB 1:500-1:1,000
  • IHC-P 1:50-1:200
  • Form: Liquid
  • Storage Condition: Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
  • Storage Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Concentration: 1ug/ul
  • Purification: Protein A affinity purified.
  • Molecular Weight: 83 kDa
  • Isotype: IgG