AnaSpec -Model AS-55152-100 -Recombinant rat MOG (1-125)
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys The sequence (Accession #CAE84068) corresponding to the extracellular domain of rat MOG along with a 6x His tag was expressed in E. coli. The recombinant rat MOG (R-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular mass of the recombinant rat MOG is 14.2 kDa.
Chemistry
- UniProt number : Q63345
- Molecular Mass/ Weight : 14.2 kDa
Modification
- Conjugation : Unconjugated
Quantity & Purity
- Concentration : 1 mg/ml
- Purification Method : Purified
- Purity : ≥95% by SDS-PAGE
Storage & stability
- Form : In solution
- Resuspension condition : rat MOG must be thoroughly mixed directly with Complete Freund’s Adjuvant (CFA). Do not dilute recombinant rat MOG with buffers that have pH greater than 4.5. Protein will precipitate at pH higher than 4.5
- Storage Buffer : 25 mM sodium acetate buffer (pH=4.0)
- Storage Conditions : Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
- Application : ELISA in vivo studies Western Blotting
- Biomarker Target : Myelin Oligodendrocyte Glycoprotein (MOG)
- Specificity : rat MOG
- Research Area : Neuroscience
- Sub-category Research Area : Multiple Sclerosis
- Usage : Research use
Source
- Host : E. coli
- Source / Species : rat