sciCONSUMABLES Scibuffers - Buffer
From sciCONSUMABLES
Discover our full range of buffers for DNA, oligos and proteins and improve the quality of your arrays. Many diagnostic assays are designed at a pH of 7.4, since this is the normal pH of human blood. However, for solid phase assays, like microarray applications, one has to distinguish between coating or spotting buffers and assay buffers. There are different buffer requirements for these two process steps.
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A spotting buffer has to guarantee:
Maximum immobilization efficiency and uniform spot morphology while also maintaining the functionality of the capture biomolecule for the downstream assay requirements.
Also, it is not all about pH; if the immobilization chemistry is based on coupling of primary amines to functional groups on the surface, amine containing buffers like Tris must be avoided. The assay buffer must guarantee optimal functionality of the involved biomolecules.
The pH value varies with buffer concentration and with temperature.
For example, the pH value of a 100 mM sodium phosphate buffer increases from 6.7 to 6.9 with 10-fold dilution.
To guarantee a sufficient buffering capacity, a concentration of 25 – 50 mM is needed in most cases.
If your assay employs enzymes which are sensitive to high ionic strength, you may try a lower concentration, e.g. 10 mM.
Did you know?
- The pH value varies with buffer concentration and with temperature.
- For example, the pH value of a 100 mM sodium phosphate buffer increases from 6.7 to 6.9 with 10-fold dilution.
- To guarantee a sufficient buffering capacity, a concentration of 25 – 50 mM is needed in most cases.
- If your assay employs enzymes which are sensitive to high ionic strength, you may try a lower concentration, e.g. 10 mM.
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