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  3. sf rabdovirus free s frugiperda cell

GlycoBac -Model Sf-RVN -Sf-Rabdovirus-Free S. Frugiperda Cell

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Sf-RVN is an Sf-rhabdovirus-free S. frugiperda cell line that can seamlessly replace the widely used, but persistently virally contaminated Sf9 or Sf21 cell lines for recombinant protein or rAAV production.

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Features of Sf-RVN cells include:

  • No Sf-rhabdovirus contamination detectable by a highly sensitive nested RT-PCR assay over more than 100 continuous passages
  • Growth rates in serum free medium identical to Sf9 Approximately 20 h doubling time in suspension culture in Expression Systems` ESF-921 medium without serum
  • Recombinant protein productivity in serum free medium identical to Sf9 Tested for three recombinant proteins
  • Higher baculovirus titers than Sf9 cells 5 to 10-fold higher levels of infectious baculovirus progeny
  • Better plaque assays than Sf9 cells Sf-RVN cells consistently produce larger, more distinct plaques in traditional plaque assays compared to Sf9 cells

Read the peer-reviewed paper describing Sf-RVN cells published in the Elsevier journal `Protein expression and purification` 

Read another peer-reviewed paper published in the Elsevier journal `Biologicals` about endogenous viral elements discovered by GlycoBac researchers in Sf-RVN and other cell lines.

GlycoBac provides Sf-RVN cells through our partner Millipore Sigma under commercial Materials Transfer Agreements (for evaluation) and/or licensing agreements (for application) in various fields of use. Please contact Dr. Jarvis directly for additional information.