Model SK-AdM096 -SmartEIA Adenovirus IgM
Enzyme immunoassay for the detection of IgM antibodies to Adenoviruses in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument. A native antigen is bound to the well surface of the microtiter plate. If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete). The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
Advantages:
- The total assay time is about 2 hours.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control and Negative Control.
- Semi-quantitative evaluation in the Index of Positivity (IP).
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
- Screening test for Adenovirus infection.
Brief assay procedure:
1.Dilute samples (1:101).
2.Incubation at 37 °C for 10 min.
3.Pipette Controls and diluted Samples.
4.Incubate at 37°C for 30 min.
5.Aspirate and wash the wells 5×
6.Pipette Conjugate.
7.Incubate at 37°C for 30 min.
8.Aspirate and wash the wells 5×
9.Pipette Substrate (TMB-Complete).
10.Incubate at 37°C for 30 min.
11.Pipette Stop Solution.
12.Read color intensity at 450 nm.
13.Evaluate the test.