SPAN - Model INC-208 - Dengue IgG/IgM Rapid Tests
The assay starts with a sample applied to the sample well. A recombinant DV antigen conjugated to colloidal gold embedded in the sample pad reacts with the DV antibody present in blood, serum or plasma forming conjugate-DV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate-DV antibody complex is captured by mouse anti human IgM-µ chain (T1) or mouse anti human IgG(T2) immobilized on a membrane forming a colored test line in the test region. A negative sample does not produce a test line due to the absence of colloidal gold conjugate-DV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of DV. A colored control line in the control region appears at the end of test procedure regardless of test result. This control line is the result of colloidal gold conjugate binding to the anti-DV antibody immobilized on the membrane. The control line indicates that the colloid
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