ExcellGene -Stabilized Wuhan Trimeric Spike Protein

This cell line is suitable for large scale manufacturing in chemically defined media. The secreted trimeric spike accumulates in the protein-free cell culture fluid of the cells. Our multi-step, HPLC-based purification process further ensures that the resulting trimeric protein is of high purity (> 95 %).

This protein is now available for applications such as diagnostics, R&D and vaccine development.

• Modifications C-terminal Transmembrane region replaced with a trimerization domain and a polyhistidine tag. Two stabilizing proline mutations and scrambled S1/S2 furin cleavage site (see reference)
• Isolate (Seq ID) Wuhan-Hu-1 (GenBank: MN908947)
• Expression system CHOExpressTMcells
• Purity > 90 % as determined by SDS-PAGE
• Molecular weight The recombinant SARS-CoV-2 trimeric spike protein consists of 3576 amino acids and predicts a molecular mass of ˜400 kDa.
• Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
• Format Liquid
• Reference Wrapp D. et al. Cryo-EM Structure of the 2019-nCoV Spike in the Prefusion Conformation. Science 367, 1260–1263 (2020)