CORALL -Total RNA-Seq Library Prep Kit

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The CORALL Total RNA-Seq Library Prep Kit enables fast and cost-efficient generation of UMI labelled, stranded libraries for whole transcriptome analyses using Illumina NGS platforms.
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  • Complete coverage of transcripts from start to end
  • Ready-to-sequence libraries within 4.5 hours
  • Unique Molecular Identifiers (UMIs) seamlessly included
  • Excellent protocol-inherent strandedness (>99 %)
  • 1 ng to 1 µg of total RNA input
  • Easy all-in-one protocol

Transcript Coverage
CORALL generates transcriptome-wide smooth, uniform read coverage, comparable to that of competitor kits.

Gene Detection
CORALL delivers excellent gene discovery rates matching conventional competitor kits. 95% of genes are commonly detected between CORALL and each of the two competitors (at >10 counts per million (CPM), Fig. 2B). This high level of library complexity ensures faithful representation of the transcriptome, enabling sensitive expression profiling.

Superior End-to-End Coverage
CORALL’s comprehensive coverage delivers improved transcript start and end site representation. Read coverage was analyzed using the ERCC spike-in controls, which feature precise, known transcription start and end sites (TSS and TES, respectively). CORALL reads map more accurately to the exact ERCC TSS than competitor libraries, which fail to cover the true start sites. Additionally, CORALL provides elevated coverage at TES.

Flexible Input
CORALL libraries can be prepared from poly(A)-enriched or rRNA-depleted RNA (100 – 200 ng of total RNA yields approximately 1 – 2 ng of rRNA-depleted RNA). CORALL is available as a stand-alone kit with single indexing or UDIs (Cat. No. 095, or 117 – 119 and 132 – 134) or in a bundle with RiboCop rRNA Depletion Kits (Cat. No. 146, 147). For mRNA analysis we recommend to use the Poly(A) RNA Selection Kit (Cat. No. 039) upstream of CORALL. Total RNA without prior depletion or enrichment (100 pg to 100 ng) can also be used as input.

Unique Molecular Identifiers (UMIs) seamlessly included
UMIs are seamlessly introduced into CORALL libraries as an inherent part of the protocol. As a result Read 1 contains the UMI information making it directly accessible in the cost-efficient single-read mode.

Data Analysis
A Data Analysis Pipeline is now available on the BlueBee® Genomics Platform. The provided pipeline enables kit users to perform read quality control, mapping, Unique Molecular Identifier (UMI) deduplication, and transcript quantification.

Enhanced Multiplexing
CORALL libraries can be multiplexed using up to 96 i7 indices (included in the single indexing kit, Cat. No. 095, 146). Additional i5 indices can also be introduced using the Lexogen i5 6 nt Dual Indexing Add-on Kits (Cat. No. 047). Used together, Lexogen’s 96 i7 and 96 i5 6 nt indices enable up to 9,216 different index combinations for sequencing.

Fast and Easy All-in-One Protocol
The streamlined RNA fragmentation-free protocol generates ready-to-sequence libraries in 4.5 hours.