Model SARS-CoV-2 IgM ELISA -Vitro Diagnostic Test
The SARS-CoV-2 IgM ELISA kit is an in vitro diagnostic test for the qualitative detection of IgM antibodies to SARS-CoV-2 in human serum. The kit is intended for use as an aid in identifying individuals with an adaptive immune response to SARSCoV-2, indicating recent or prior infection. At this time, it is unknown for how long antibodies persist following infection and if the presence of antibodies confers protective immunity.
The SARS-CoV-2 IgM ELISA KIT should not be used to diagnose acute SARS-CoV-2 infection. Results are for the detection of IgM SARS-CoV-2 antibodies.
IgM antibodies to SARS-CoV-2 are generally detectable in blood several days after initial infection, although the duration of time antibodies are present post-infection is not well characterized. Individuals may have detectable virus present for several weeks following seroconversion.
The sensitivity of SARSARS-CoV-2 IgM ELISA early after infection is unknown. Negative results do not preclude acute SARS-CoV-2 infection. If acute infection is suspected, direct testing for SARS-CoV-2 is necessary.
False positive results for SARS-CoV-2 IgM ELISA may occur due to cross-reactivity from pre-existing antibodies or other possible causes.
- SARS-CoV-2 Antigen Coated Microtiter Plate: 1×96 well
Strip holder in a resealable foil pouch, containing 96 polystyrene microtiter wells coated with SARS-CoV-2 antigen in each well.
- Sample dilution Buffer (DIL): 2×15,5 mL
Sample Dilution Buffer will be used for the dilution of test samples and controls. Phosphate buffer pH 7.40, NaCl, KCl, Proclin 300, Interference masking agent
- 100X Secondary Conjugate Ab: 12 mL
Horseradish peroxidase-labeled secondary antibody. Phosphate buffer pH 7.40, NaCl, KCl, Proclin 300.
- Secondary Ab dilution Buffer: 1×12 mL
Phosphate buffer pH 7.40, NaCl, KCl, Proclin 300 Stable at 2-8°C until the expiration date.
- 5X Wash Buffer (WS): 1×100 mL
5X concentrated buffer. Phosphate buffer pH 7.40, NaCl, KCl, Tween 20, Proclin 300
- TMB Substrate: 1×12 mL
TMB chromogenic substrate. 3,3,5,5′-Tetramethylbenzidine.
- Stop Solution: 1×12 mL
Stop Solution. Sulfuric Acid 1 – 4%