Antibodies and Immunoassays
From Research
Our team of scientists performs integrated research, development, and technical support to market the antibodies against S-adenosylmethionine, or the SAM molecule. We are dedicated to develop several formats of immunoassays to detect SAM and S-adenosylhomocysteine (SAH) from biosamples efficiently. Immunoassays have been approved to be easy to use without requiring costly equipment, accurate, sensitive, specific and fast. With our antibodies one can determine the concentration of the SAM molecule in samples without costly equipment. Arthus Biosystems of Richmond, California, offers a complete series of research tools for research laboratories worldwide to measure SAM effectively using immunoassay technology.
An Important Research Tool
Antibodies are known to be very useful in the process of discovering many biological aspects of the specific antigens with which the antibodies bind to specifically, including quantification, qualification, localization, diagnostics, etc. Commonly used areas are Enzyme Linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC) , Immunofluorescence (IF), Flow Cytometry(FCM), Immunoprecipitation (IP), Western Blot, Dot Blot, Enzyme Linked Immunospot (Elispot), Fluorescence Activated Cell Sorting (FACS). Scientists have not had any anti-SAM antibody available until now. With our unique series of products, more and more important scientific findings relating to SAM methylation, transsufuration, aminopropylation in important biological processes including DNA repair, carcinogenesis, neurodegeneration, tissue differentiation,inflammation,aging,general pathogenesis will be revealed.
Antibodies against SAH
We have successfully generated several mouse monoclonal antibodies against S-adenosylhomocysteine (SAH). Compared with other SAH monoclonal antibodies, our mouse monoclonal antibody against SAH has a better sensitivity, can detect free SAH molecule, and can be used for measuring SAH from bio-samples.
Our products make it possible to measure the levels of S-adenosalmethionine and and S-adenosylhomocysteine at the same time in biosamples quickly and easily by common technicians in a lab with inexpensive instruments. This opens a door for the research communities to effectively measure methylation index in a variety of their research areas.
Immunoassays
Direct and indirect ELISA to quantitatively measure SAM and SAH are developed. The sensitivity from ELISA procedure for SAM can reach as low as a few nanomolar/liter. Other forms of quantitative and qualitative immunoassays are also being developed.
Clinical observations
By using quick, easy and convenient immunoassays, clinical labs and research labs are now able to perform more clinical observations on how SAM, SAH and methylation index relate to diseases and health status. We have measured SAM, SAH levels from plasma, serum and saliva samples from normal subjects and patients. Some results are summarized in here. Elevated serum levels of S-adenosylhomocysteine, but not SAM and homocysteine, are associated with cardiovascular disease in stage 5 chronic kidney disease patients (Clin Chim Acta. 2008; 395:106). More systematic studies are underway, which will speed up the process of properly applying these biomarkers in clinical practice and help assist in usage of SAMe as a medicine.
Customer reviews
No reviews were found for Antibodies and Immunoassays. Be the first to review!