Digital Pathology Image Analysis Services
To accurately interpret gene expression in tissues, scientists are in need of a quantitative assay that provides spatial information at the molecular level. The RNAscope™, BaseScope™, and miRNAscope™ ISH technologies enable single molecule detection of RNAs in situ within the tissue context. Our highly trained scientific experts and pathologists offer various quantification methodologies. The analyses are defined according to the biological question being addressed, and each project is supported by consultations with the scientific team.
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Digital Image Analysis
- Quantitative cell-by-cell gene expression data
- Identify tissue types or regions of interest
- Optional heat maps provide full-tissue spatial expression
Image analysis is performed using HALO™ (Indica Labs), the powerful digital pathology tool for quantitative gene expression analysis. HALO reports morphological and multiplexed gene expression data on a cell-by-cell basis across entire tissue sections, and is applicable for RNAscope, BaseScope, and miRNAscope singleplex, duplex, and multiplex fluorescence assays. Sorting and filtering capabilities allow the user to mine millions of cells while visually assessing the corresponding cells in situ. The software can be trained to classify cell populations or regions of interest (ROI), for example categorizing a tissue into tumor cells versus stromal cells.
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