MtoZ Biolabs -Model mrna-3-poly-a-tailing-analysis -mRNA 3’ poly(A) Tail Analysis Service
In eukaryotic cells, the 3' end of mRNA is often extended with a poly(A) tail, a sequence of purine nucleotides. The poly(A) tail is a long chain of adenosine nucleotides of variable length. This tail is crucial for mRNA stability, transport, and translation. The analysis of mRNA's 3' poly(A) tail is a vital technique in studying the polyadenylation at mRNA molecules' 3’ ends, with significant implications for gene regulation, cell development, and disease research. This analysis aids in understanding mRNA processing, stability, transport, and post-transcriptional regulatory processes. Additionally, it is instrumental in drug development and disease diagnostics, enhancing our understanding of disease mechanisms and informing therapeutic strategy design.
Gilar, M.et. al. Anal. Chem. 2023.
Figure 1. mRNA Molecule With a 3’ Poly(A) Tail
Utilizing endonuclease and liquid chromatography-mass spectrometry (LC-MS) technologies, the analysis includes RNAse T1 enzyme digestion and oligo dT magnetic bead purification to isolate the poly(A) tail, followed by qualitative and quantitative assessment via LC-MS. This approach is also a commonly used polyA tailing analysis method in the current in vitro mRNA preparation process. MtoZ Biolabs employs the Orbitrap Fusion Lumos mass spectrometer and Nano-LC chromatography to offer precise, comprehensive mRNA 3’ poly(A) tail analysis services. Free project evaluation, Welcome to learn more details!
MtoZ Biolabs provides a detailed technical report, which includes:
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Comprehensive mRNA 3’ Poly(A) Tail Analysis
4. Mass Spectrometry Images
5. Raw Data
MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
Email: marketing@mtoz-biolabs.com