Shallow Shotgun Sequencing Services & Solutions
From Sequencing Services
Through extensive R&D, the CosmosID team have established optimum read depth ranges for different samples types, using species and strain-level Bioinformatics. Whilst the optimum depth for any given sample will depend on study intent, microbial diversity & expected host DNA contamination, CosmosID offers shallow shotgun sequencing at 3M reads which yields consistent species and strain-level resolution of bacteria.
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Is shallow shotgun right for you?
With Shallow Shotgun Sequencing, researchers can unlock the microbiome with better species level resolution than 16S sequencing whilst also maintaining cost effectiveness, making it a fantastic option for large cohort studies that focus on the gut microbiome where statistical significance is paramount!
Advantages of shallow shotgun sequencing include:
- Cost-effectiveness
- Well-suited for biobanking, large cohort & population studies
- Well-suited for gut microbiome research
- Well-suited for routine testing, including microbiome-based diagnostics
CosmosID’s CLIA-certified, GCP compliant facility receives samples from all around the world. Your precious pre-clinical or clinical samples will be barcoded & accessioned into the CosmosID LIMS system for sample tracking and project management.
CosmosID also offers validated DNA extraction protocols which are optimized by sample type, resulting in minimal bias, optimum yield and purity.
Once DNA QC metrics are established for received or generated DNA samples, these samples then undergo Illumina library preparation and QC prior to sequencing at 3 Million total reads (1X150bp or 2X150).
In addition to sample processing & sequencing, CosmosID includes, as standard:
- Multi-kingdom species & strain-level resolution via the CosmosID Metagenomics App
- Access to AMR & Virulence genes
- Interactive and user-friendly charts & visualizations with access to comparative analysis features including heat maps, PCA plots, Alpha Diversity & Beta Diversity PCoA
- Methods & QC data upon request – DNA QC, Library QC & fastQC
- Fastq/Fasta file transfer via AWS s3 Bucket/Dropbox
CosmosID offers different turnaround time options as fast as 3-5 business days for shotgun sequencing & analysis. Simply let us know about any special requirements and we can tailor our service accordingly.
Once samples have arrived at the lab, our operations team will keep you updated on a weekly basis on the status of your study!
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