Shotgun Metagenomics Services
At Cmbio, we offer both shallow and deep shotgun sequencing options to suit the specific requirements of your study. Whether you're conducting exploratory research, clinical trials, or large-scale population studies, our services are designed to deliver accurate, high-quality data that empowers your research.
Ideal for large cohort studies and projects where cost-effectiveness is crucial, our shallow shotgun sequencing service provides consistent species and strain-level resolution of bacteria at a sequencing depth of approximately 3 million reads per sample. This approach offers better resolution than traditional 16S rRNA gene sequencing and is particularly well-suited for gut microbiome research.
Advantages of Shallow Shotgun Sequencing:
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Cost-Effective: Enables large-scale studies without compromising data quality.
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Enhanced Resolution: Provides species-level identification with improved diversity detection across multiple kingdoms, including bacteria, viruses, fungi, and protists.
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Suitable for Low Biomass Samples: Effective for samples with low microbial content, such as swabs.
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Ideal for Routine Testing: Supports microbiome-based diagnostics and routine surveillance.
For projects requiring extensive coverage and detailed characterization of microbial communities, our deep shotgun sequencing service offers sequencing depths ranging from 6 million to over 100 million reads per sample. This approach builds upon the benefits of shallow shotgun sequencing, providing additional advantages for in-depth analysis.
Advantages of Deep Shotgun Sequencing:
In addition to all the benefits of shallow shotgun sequencing, deep shotgun sequencing offers:
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Higher Resolution: Achieves species, subspecies, and strain-level identification with greater accuracy.
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Enhanced Multi-Kingdom Profiling: Provides comprehensive profiling of bacteria, viruses, bacteriophages, fungi, and protists with increased sensitivity.
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Detailed Functional Insights: Enables characterization of antimicrobial resistance genes, virulence factors, plasmids, and functional pathways at a deeper level.
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Improved Detection of Low-Abundance Organisms: Increased sequencing depth enhances the detection of rare or low-abundance microorganisms and genes.
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De Novo Strain Profiling: Enables engraftment and strain tracking analysis, essential for probiotic- and FMT studies.
Human Health and Disease Research
Explore the role of the microbiome in various health conditions.
Clinical Trials and Pharmaceutical Studies
Analyze clinical samples for primary or exploratory endpoints.
Biomarker Discovery and Patient Stratification
Identify microbial markers for drug development and personalized medicine.
Epidemiology and Strain Tracking
Monitor the spread of pathogens and antimicrobial resistance.
Infectious Disease Diagnostics
Support clinical metagenomics for rapid pathogen identification.
Fecal Microbiota Transplantation (FMT)
Screen donor and recipient samples for safety and efficacy.
Agricultural and Environmental Research
Investigate microbial communities in soil, water, and other environmental samples.
Food Safety and Spoilage Studies
Detect and characterize microbial contaminants in food products.