DILIsym - Software for Predicting Drug-Induced Liver Injury

DILIsym application examples include

Important DILIsym application examples include:

• Comparison of ubrogepant with prior in-class molecules for DILI potential, which was included in an FDA review of the ubrogepant safety data
• Comparison of lixivaptan’s liver safety profile with tolvaptan, which was submitted to the FDA as part of the new IND application for lixivaptan
• Mechanistic analysis of acetaminophen’s carcinogenic potential, which was submitted to the California Office of Environmental Health Hazard Assessment (OEHHA) regarding Proposition 65 as part of the weight of evidence suggesting no cancer risk; FDA agreed
• Mechanistic analyses of the liver safety of remdesivir, tolvaptan, pexidartinib, and TAK-875
• Comparison of the liver safety mechanisms of several macrolide antibiotics as part of the solithromycin new drug application advisory committee meeting
• Liver safety biomarker analyses for entolimod and GGF2
• Dose optimization for a novel antibiotic, BAL30072

DILIsym integrations

DILIsym simulations integrate compound exposure with compound mediated mechanisms of intrinsic drug toxicity, and with inter-individual variability. The intersection of these is prediction of hepatotoxicity for:

• Phase II, III, or IV clinical trials in patients
• First-in-human clinical trials in healthy volunteers
• Small preclinical species
• Large preclinical species

DILIsym is a “middle-out”, multi‑scale representation of drug-induced liver injury. It includes key liver cell populations (e.g., hepatocytes, Kupffer cells), bile duct cells (cholangiocytes), intracellular biochemical systems (e.g., mitochondrial function), and whole body dynamics (e.g., drug distribution and metabolism). DILIsym represents these biochemical and physiological processes for mice, rats, dogs, and humans, based on a substantial amount of publicly-available literature. Through the generation of SimPops® (a collection of simulated patients forming a simulated population), DILIsym also includes inter‑individual variability.

DILIsym includes a graphical user interface (GUI) to facilitate in silico investigations. The GUI permits the specification of new simulated experiments, including compound, dose, dosing frequency, duration, mechanism(s), and species. Simulations can be performed on individuals or populations. Simulation results can be visualized within the GUI or exported for further analysis in other software.  Simulations can also be run with the command-line version of DILIsym, making integration into a stream-lined workflow possible.  A high performance grid license (HPGL) option is available via licensing (or included with DILI-sim Initiative membership), which allows for use of an unlimited number of command-line-based simulation engines and enables users to scale up and complete large batches of simulations on internal or external compute clusters.

DILIsym prospectively supports key management decisions by providing information about potential drug-induced liver injury risk in upcoming experimental or clinical study designs, as well as mechanistic rationale for the underlying biochemical events that could cause liver toxicity. DILIsym also supports key management decisions about compounds for which liver transaminase elevations have already been observed by identifying patient screening or dosing protocols to mitigate risk. Additionally, DILIsym can mechanistically interpret existing data on liver transaminase elevations to predict the underlying level of hepatocyte loss and rate of liver recovery, supporting the distinction of innocuous vs. dangerous liver signals.

Conceptual framework for DILIsym development

DILIsym has been developed within a conceptual framework (Figure 1), wherein liver compound exposure (parent and/or metabolite) can engage various mechanisms of intrinsic toxicity. The mechanisms currently included are: (1) inhibition of bile acid transporters leading to disruptions in bile acid homeostasis, (2) alteration of mitochondrial function, (3) generation of oxidative stress, and (4) cholestatic injury to bile duct cells via disruption in the ratio between phospholipids and bile acids in the bile.  Alone or in combination, these mechanisms can lead to hepatocyte cell death via apoptosis or necrosis. Hepatocyte necrosis releases damage associated molecular pattern (DAMP) molecules, capable of activating innate immune cells (e.g., resident Kupffer cells). Activated immune cells produce mediators that may serve to amplify the ongoing injury and/or may promote liver regeneration. Immune cells may also accumulate due to ongoing inflammatory processes. Importantly, while the various elements described thus far can be sufficient to drive liver injury, they may also provide the setting in which an adaptive immune response can develop. Adaptive immune responses are not currently included in DILIsym but are part of ongoing development to be included in future versions of the modeling software.

What DILIsym includes?

DILIsym includes multiple sub-models which are solved in an integrated fashion to generate simulation results. Sub-models include intracellular biochemical systems that may be impacted by compound exposure (e.g., bile acid homeostasis, mitochondrial function, oxidative stress), the hepatocyte and cholangiocyte life cycles, and stimulation of innate immune responses. To facilitate comparison with high-level clinical and preclinical readouts, DILIsym includes the release of biomarkers (e.g., ALT, AST, ALP) from hepatocytes in the event of cell death, as well as increases in total bilirubin, reflecting compromised liver function due to hepatocyte loss or direct bilirubin-processing enzyme/transporter compound effects. DILIsym also includes emerging biomarkers (e.g., GLDH, miR-122, CK-18) that may provide additional mechanistic information. More detailed descriptions of the sub-models are provided in the links below. Specific data used in design and development are available to licensees through the DILIsym modeling software documentation.

Finally, DILIsym includes SimPops, comprising simulated individuals expressing variability in key parameters related to susceptibility to various mechanisms of toxicity. SimPops permit the researcher to consider the effect of inter-individual variability on drug toxicity.