Serum Products for Mesenchymal Stem Cells Growth - Medical / Health Care
Growth performance of human adipose-derived mesenchymal stem cells in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 2% SimPL (left). Similarly, cells were grown in identical conditions except for the use of reduced-serum basal media (Gibco™ Advanced DMEM/F12) with either 2% FBS or 2% SimPL (right). Medias supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 1.7 mM Mg. Prior to growth evaluation, cells were passaged using standard techniques in 10% FBS with standard DMEM/F12 basal media and were seeded into each condition without adaptation into a 96-well plate.
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Data represent cell counts relative to the initial seeding density average of each condition obtained from label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader.
Reduced-Serum Basal Media
Growth performance of human adipose-derived mesenchymal stem cells in media containing Simplified Platelet Lysate (SimPL) and fetal bovine serum (FBS). Cells were grown in standard DMEM/F12 basal media containing penicillin, streptomycin, and GlutaMAX™ with either 10% (v/v) FBS or 2% SimPL (left). Similarly, cells were grown in identical conditions except for the use of reduced-serum basal media (Gibco™ Advanced DMEM/F12) with either 2% FBS or 2% SimPL (right). Medias supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 1.7 mM Mg. Prior to growth evaluation, cells were passaged using standard techniques in 10% FBS with standard DMEM/F12 basal media and were seeded into each condition without adaptation into a 96-well plate. Data represent cell counts relative to the initial seeding density average of each condition obtained from label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader.