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Application of refractable nanocomposite on cell based therapy in central nervous system
Courtesy of Inderscience Publishers
Quantum Dots (QDs) are nano–particles carrying high stability fluorescence to follow the grafted cells which can be used in investigating the movement of cells and understanding the organogenesis of stem cells during development and wound repair processes in adulthood. For its easy acquirement and bypassing moral ethical issues, it makes the human adipose derived stem cells an attractive, alternative source for clinical cell therapy. When labelled with fluorescence dye, the location of injected stem cells can be monitored. In order to determine the behaviour and location of transplanted human adipose derived stem cells, the QDs labelled stem cells are implanted by local injection at thoracic 8 spinal cord in rats in this study. The expression of QDs in adult rats is evaluated at 2 hours, 1, 7 and 14 days after implantation. The results show the QDs labelled human adipose derived stem cells can be observed to trace implanted cells in vivo at least 14 days, and the data of this study provide the feasible analysis on stem cell based therapy applied in central nerve system repair.
Keywords: quantum dots, refractable nanocomposites, cell therapy, central nervous system, human adipose derived stem cells, fluorescence, spinal cord, in vivo, nanotechnology, nanoparticles, nerve repair
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