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Assay Articles & Analysis
251 articles found
Researchers often use extracellular domains for ligand-binding studies. For signaling assays, mammalian expression systems are preferred to ensure functional receptor conformation. ...
Techniques such as T7 endonuclease I assays or Surveyor assays can measure the frequency of indel formations, which indicate successful editing. ...
Utilizing FFPE blocks derived from the rat thymus enables researchers to conduct various assays, including immunohistochemistry, in situ hybridization, and gene expression studies. ...
In preclinical respiratory studies, traditional methods like plethysmography often require animal restraint and extensive acclimation, limiting their ability to capture dynamic respiratory changes. To overcome these challenges, IPS Therapeutique Inc. validated a novel telemetry-based technique for continuous monitoring of intrapleural pressure and respiratory patterns in conscious, freely ...
The recombinant form of ELANE protein is typically expressed in eukaryotic systems to preserve post-translational modifications and enzymatic activity. Activity assays, such as chromogenic peptide cleavage or fluorogenic substrate kinetics, are used to validate protein functionality post-purification. ...
The carboxylated version is ideal for binding studies and functional assays, whereas non-carboxylated MGP may be used for antibody generation, epitope mapping, or as a control in mechanistic investigations. ...
Researchers can perform various assays to evaluate podocyte health, migration, differentiation, and interaction with other renal cell types. ...
This capability is assessed through spontaneous differentiation assays, where iPSCs are cultured in conditions that promote differentiation into various cell types, such as neurons, cardiomyocytes, or hepatocytes. ...
Streamlined Process for Drug Development The screening process typically involves several steps designed to ensure comprehensive analysis and reliable results. Initial assays are developed to evaluate the activity of kinases against a library of potential inhibitors. ...
These include: ELISA (Enzyme-Linked Immunosorbent Assay): This is widely used to assess antibody binding to specific antigens present in different tissues. ...
Beyond therapeutic uses, recombinant proteins are instrumental in research and diagnostics. They serve as tools in assays, studies of protein interactions, and the development of novel research methodologies. ...
One of the most widely recognized in vitro models for evaluating the intestinal absorption of compounds is the Caco-2 permeability assay. This assay plays a pivotal role in understanding how drugs are absorbed in the gastrointestinal tract and aids in predicting their bioavailability. ...
Comparing gold nanoparticles with latex beads, it can be observed that lateral flow assays using latex beads on the conjugation pad have higher sensitivity and specificity than assays using gold nanoparticles. ...
By utilizing the S9 fraction in assays, researchers can simulate the metabolic processes that a drug will encounter in a living organism. ...
The MDCK permeability assay is a critical technique employed in pharmacology and drug development to evaluate the permeability of drug candidates across epithelial cell layers. ...
Thin sections facilitate the visualization of cellular details, while thicker sections may be preferred for certain types of assays. Applications of Frozen Lung Sections Once prepared, frozen lung sections can be utilized for various applications: Histological Analysis: Staining techniques, such as Hematoxylin and Eosin (H&E) staining, allow researchers to visualize lung ...
This is typically performed using techniques like flow cytometry and radiolabeled binding assays. 3. Tumor Inhibition Potency Analysis ServiceOnce binding characteristics are established, the next step is to assess tumor inhibition potency. ...
Diagnostics: Their high specificity and stability make them ideal candidates for diagnostic assays, including imaging and biosensors. Research Tools: Researchers utilize single-domain antibodies in various applications, such as protein purification and label-free detection systems, due to their ability to bind specific targets with high affinity. ...
Techniques such as mass spectrometry, analytical HPLC, and functional assays are employed to assess the structure, efficacy, and safety of the ADC. ...
High Sensitivity and Specificity: Utilizing advanced multiplex assays, the Rat Cytokine and Chemokine Panel ensures reliable and reproducible results, even at low concentrations of analytes. ...