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Cell Isolation Articles & Analysis

21 articles found

Single-Domain Antibody Production: A Comprehensive Overview

Single-Domain Antibody Production: A Comprehensive Overview

They can be produced in microbial systems such as yeast or bacteria, which reduce the complexity and cost associated with mammalian cell culture. Reduced Immunogenicity: Due to their smaller size and unique structure, single-domain antibodies tend to elicit a lower immune response in humans, making them safer for therapeutic applications. ...

ByCreative Biogene


New Approach to Successfully Purify and Characterize Human Neural Stem Cells

New Approach to Successfully Purify and Characterize Human Neural Stem Cells

Fluorescence-activated cell sorting is used to isolate cell types in brain tissue from single-cell suspensions based on cell surface immunophenotype. ...

ByCreative Bioarray


Mitochondrial Protein Sequencing

Mitochondrial Protein Sequencing

Mitochondria are a unique type of organelle present within cells, serving as the primary source of energy within living organisms. ...

ByMtoZ Biolabs


T Cell Antigen Receptor Sequencing

T Cell Antigen Receptor Sequencing

The T cell antigen receptor (TCR) is a characteristic marker of T cells, playing a crucial role in the immune system. Each T cell typically expresses a specific TCR, which has a unique structure and sequence that can recognize different antigens, including bacteria, viruses, tumor cells, and other pathogens. ...

ByMtoZ Biolabs


AI and Multiplex Cloning Redefine Aantibody Discovery

AI and Multiplex Cloning Redefine Aantibody Discovery

The Latest Technological Advances in Antibody Discovery1. Single B Cell Isolation and SequencingThe isolation and sequencing of single B cells can help us gain a deeper understanding of the diversity and specificity of B cells. ...

ByMtoZ Biolabs


Cytokines in CAR-T Cell Therapy

Cytokines in CAR-T Cell Therapy

What is CAR T-cell therapy? Car-t cell therapy involves genetically engineering T cells isolated from patients or allogeneic donors to express chimeric antigen receptors (CAR) that specifically recognize and kill tumor cells. ...

ByCreative Proteomics


New Approach to Successfully Purify and Characterize Human Neural Stem Cells

New Approach to Successfully Purify and Characterize Human Neural Stem Cells

Fluorescence-activated cell sorting is used to isolate cell types in brain tissue from single-cell suspensions based on cell surface immunophenotype. ...

ByCreative Bioarray


Exploring the Potential of RNA-Seq in Various Fields

Exploring the Potential of RNA-Seq in Various Fields

Pathway analysis and co-expression network analysis are two important included parts. Single-cell RNA-seq Single-cell RNA-seq is a way to find out how internal cell processes and outside stimuli work together to decide the fate of a cell.It also contributes to a better understanding of how an ‘outlier cell’ may ...

ByCD Genomics


The University of Chicago - Case Study

The University of Chicago - Case Study

The research goals of Carole Ober’s laboratory at the University of Chicago are to identify genetic variants that influence gene expression and epigenetic patterns in tissues relevant to complex phenotypes, especially related to asthma and fertility. The lab uses both freshly isolated cells, as well as tissue and cell culture models of ...

ByAdvaita Bioinformatics


What Is Single-Cell Sequencing and How to Achieve It?

What Is Single-Cell Sequencing and How to Achieve It?

With the development of sequencing technology, as well as cell isolation and whole genome amplification, single-cell sequencing technology, which sequences genomes at the level of individual cells, has gradually emerged to solve the problem of inaccessibility of microsamples for detection on the one hand, and to better resolve ...

ByCD Genomics


Viable SARS-CoV-2 Delta variant detected in aerosols in a residential setting with a self-isolating college student with COVID-19

Viable SARS-CoV-2 Delta variant detected in aerosols in a residential setting with a self-isolating college student with COVID-19

SARS-CoV-2 detected in an area of a single-family dwelling distant from an isolation suite occupied by COVID-19 patient. Assessment of air samples and surface swab samples by rRT-qPCR and cell culture for SARS-CoV-2. ...

ByAerosol Devices Inc.


Cell Transplantation Promising for Treatment of Biliary Diseases   

Cell Transplantation Promising for Treatment of Biliary Diseases  

When liver regeneration is severely impaired in mice, BECs can develop into hepatocytes or bile duct cells. This bipotential cell population can be isolated, expanded, and transplanted to repopulate the damaged liver soft tissue and differentiate into mature hepatocytes in order to restore liver function when endogenous regenerative mechanisms ...

ByCreative Biolabs


Researchers Prepared New Tools for the Production of Exosome Drug Carriers

Researchers Prepared New Tools for the Production of Exosome Drug Carriers

In the study, the clinically approved anti-tumor drug doxorubicin was selected as the validation object, which confirmed that the "exosome nanoperforator" could efficiently load it into the exosome, and the drug-loaded exosome could transport doxorubicin into lung cancer cells and tumor spheres, induce cancer cell death and inhibit tumor sphere growth. ...

ByCreative Biostructure


The Power Of RepliGut in Vitro Intestinal Stem Cell Models

The Power Of RepliGut in Vitro Intestinal Stem Cell Models

Since we obtain the entire organ, it is also possible to isolate donor-matched immune cells, endothelial cells, epithelial cells, fibroblasts, and fecal microbiota from a single donor to build a comprehensive model. A key feature of our biobank is our proprietary methods to isolate and generate large ...

ByAltis Biosystems


Recapitulating the Multifaceted Inflammatory Environment of the Intestine In Vitro

Recapitulating the Multifaceted Inflammatory Environment of the Intestine In Vitro

As expected, cells isolated from donors behave slightly differently; however, similar trends are observed among all. ...

ByAltis Biosystems


Biorep Innovation Partner to COVID-19 Stem Cell Therapy Trial

Biorep Innovation Partner to COVID-19 Stem Cell Therapy Trial

The therapy uses cells from discarded umbilical cords, with just one cord saving as many as 10,000 lives. ...

ByBiorep Technologies, Inc.


Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota

Effects of oral administration ofL. rhamnosus KBL365 orL. paracasei KBL382 on Treg in mesenteric lymph nodes (MLNs) of AD mice. The MLN cells isolated from NC/Ng mice in Control, DFE+PBS, KBL365-treated, and KBL382-treated mice. (A and B) The number of CD4+ CD25+ Foxp3+ cells was calculated based on the percentage of total MLN ...

ByKoBioLabs


Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells

Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells

Abstract We previously generated 32 rotavirus-specific (RV-specific) recombinant monoclonal antibodies (mAbs) derived from B cells isolated from human intestinal resections. Twenty-four of these mAbs were specific for the VP8* fragment of RV VP4, and most (20 of 24) were non-neutralizing when tested in the conventional MA104 cell–based ...

ByJohnson Controls, Inc.


Cells isolated from donors with nonalcoholic fatty liver disease exhibit disease phenotypes in 3D bioprinted human liver tissue

Cells isolated from donors with nonalcoholic fatty liver disease exhibit disease phenotypes in 3D bioprinted human liver tissue

The characteristics of donors from which the cells are isolated, and especially their stage on the NAFLD continuum, are likely to influence the resulting performance in two-dimensional (2D) and 3D models. ...

ByOrganovo Holdings Inc.


Accelerating stem cell knowledge into regenerative medicine by OUH-CELL-BENCH

Accelerating stem cell knowledge into regenerative medicine by OUH-CELL-BENCH

One major challenge for implementing stem cell treatment in the clinics, is the interdisciplinary work required by stem cell biologists and medical doctors as well as the state-of-the-art equipment needed at public hospitals to successfully translate the basic stem cell knowledge into clinical products. Our facility directly serves to speed up ...

ByBaker

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