clinical sample Articles
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Borrelia burgdorferi sensu stricto is clonal in patients with early lyme borreliosis
Lyme borreliosis, the most commonly reported vector-borne disease in North America, is caused by the spirochete Borrelia burgdorferi. Given the extensive genetic polymorphism of B. burgdorferi, elucidation of the population genetic structure of the bacterium in clinical samples may be relevant for understanding disease pathogenesis and may have applicability for the development of diagnostic ...
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Direct Detection of Unamplified Hepatitis C Virus RNA Using Unmodified Gold Nanoparticles
Abstract Background:Gold nanoparticles (AuNPs) exhibit a unique phenomenon known as Surface Plasmon Resonance,which is responsible for their intense red color. This color changes to blue upon aggregation of AuNPs. Objective:Thiswork aims to develop a rapid, simple and cheap assay for direct detection of unamplified HCV RNA extracted from clinical samples using unmodified AuNPs. Methods:Serum ...
By D-Kimia, LLC
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PCR assay targeting virulence genes of
Helicobacter pylori isolated from drinking water and clinical samples in Lahore metropolitan, PakistanHelicobacter pylorus is considered for chronic gastritis, gastric ulcers and adenocarcinoma and its high infection rate is observed in overcrowded and lower socioeconomic groups in developing countries. This study was designed to identify the role of drinking water in the transmission and prevalence of H. pylori (HP). Selective HP medium was developed for enrichment and presumptive identification ...
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UCT Silane Cited in Clinical Microbiology
Fungal bloodstream infections have been noted as a significant problem in many countries including the United States, with an attributable mortality rate of up to 40%. An early diagnosis to direct appropriate therapy has been shown to be critical to reduce mortality rates. Conventional phenotypic methods for fungal detection take several days, which is often too late to impact outcomes. The ...
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The National Cancer Centre Singapore - Case Study
Name: Ms. Kam Siok Yuen Job Title: Research Officer Company: The National Cancer Centre Singapore The National Cancer Centre Singapore HQ [Peak Protected] keeps National Cancer Centre in Singapore lab running The National Cancer Centre in Singapore is a research and treatment facility for cancers which are most commonly found in those of Asian descent. With three main research divisions, ...
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Targeted Capture and Sequencing: An Accurate and Efficient Method for Human Pathogen Detection
Next-Generation Sequencing or NGS has provided scientists alternative approaches in understanding DNA. The technology has come a long way from the simple sequencing technique developed by Sanger in 1977 and continuous improvements are being done to better cater to the needs of researchers. One of the recent advances to NGS is Target-and-Capture Sequencing or Capture-Seq. Capture-Seq allows the ...
By CD Genomics
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The Workflow of Viral Metagenomics
As is known to all, most of the viruses infect microorganisms, plants, and animals. They cause familiar infectious diseases (such as the flu and warts) and even some severe illnesses such as smallpox, Ebola, and HIV/AIDS. Because less than one percent of microbial hosts have been cultivated, it is very important to identify and measure the community dynamics of viruses in the environment. Unlike ...
By CD Genomics
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Sensitivity of antibiotic resistant and antibiotic susceptible Escherichia coli, Enterococcus and Staphylococcus strains against ozone
Tolerance of antibiotic susceptible and antibiotic resistant Escherichia coli, Enterococcus and Staphylococcus strains from clinical and wastewater samples against ozone was tested to investigate if ozone, a strong oxidant applied for advanced wastewater treatment, will affect the release of antibiotic resistant bacteria into the aquatic environment. For this purpose, the resistance pattern ...
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Targeted Region Sequencing in Human Disease Studies and Clinical Care
Introduction to target region sequencing Since the introduction of Sanger sequencing in 1977, genetic sequencing has been greatly improved with costs simultaneously falling. With the ability to rapidly produce large volumes of sequencing data, next-generation sequencing (NGS) enables researchers to obtain whole genome or targeted regions of samples. Targeted region sequencing is preferred by ...
By CD Genomics
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Generating a robust prediction model for stage I lung adenocarcinoma recurrence after surgical resection
Abstract Lung cancer mortality remains high even after successful resection. Adjuvant treatment benefits stage II and III patients, but not stage I patients, and most studies fail to predict recurrence in stage I patients. Our study included 211 lung adenocarcinoma patients (stages I–IIIA; 81% stage I) who received curative resections at Taipei Veterans General Hospital between January ...
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Clinical Research On-Demand: How the University of Washington’s Theberge Lab is Using Tasso to Study Unpredictable Events in Real Time - Case Study
Many important research questions cannot be studied under controlled circumstances. Events like natural disasters or disease exposure often occur without warning and prevent in-person clinic visits, making timely observation difficult without a flexible approach. At the University of Washington, researchers are partnering with Tasso to study these scenarios by deploying on-demand remote ...
By Tasso, Inc.
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Protein Quantification Techniques and Their Application in Clinical Research
Representing the expression, post-translational modification, and protein-protein interaction for certain cells, tissues, or organs, proteomics has become an increasingly important means to understand the molecular mechanism and signal transduction of human disease and cancer. Protein levels in the body are in a steady state of equilibrium. Accumulation of proteins usually leads to qualitative ...
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No-touch Forehead Temperature Measurement in Two Clinical Settings
Background. Healthcare personnel prefer devices that maximize efficiency while minimizing cross-contamination as well as cost. The Caregiver® (Thermomedics, Inc., Miami. FL), a new infrared non-contact forehead thermometer has been developed for clinical professional use. Our objective was to evaluate the clinical accuracy and repeatability of the Caregiver as compared to measurements ...
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Applications of Metagenomics in Biotechnology and Health Care
Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental ...
By CD Genomics
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Applications of Metagenomics in Biotechnology and Health Care
Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental ...
By CD Genomics
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Applications of Single-Cell RNA Sequencing in Drug Discovery and Development
Advantages of Single-Cell RNA Sequencing in Drug Discovery Single-Cell RNA Sequencing (scRNA-seq) offers several advantages in drug discovery, primarily through target identification. By analyzing complex tissues and diseases at the cellular level, scRNA-seq allows researchers to identify distinct cell subtypes and their gene expression profiles. This knowledge enhances our understanding of ...
By CD Genomics
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Set Up Your Clinical Trial For Success During The Holidays
The Holiday season often brings mixed feelings for many. While it can be a time of relaxation with friends and family for some, for others, preparing for this time means ensuring business continuity despite a typical slow down. This can be a source of undue stress if it is not suitably planned for. ...
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Amplicon Sequencing for Microbiome Research: Introduction, Advantages, and Applications
The identification of microbial groups is an important step in understanding the interrelationship of microorganisms in a community. It is done to determine all present microbial taxa and their relative abundance, and as well their phylogenetic relationships. One of the methods used to establish phylogenetic relationships is by sequencing common genes. One of the easiest genes to be sequenced are ...
By CD Genomics
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Novel Mechanism of Glycosylation Regulating Pancreatic Cancer Reveled
Pancreatic cancer is a highly malignant digestive system tumor, and the five-year survival rate of patients after diagnosis is only about 10%. Changes in metabolism are one of the important features of tumor cells. Tumor cells undergo metabolic reprogramming to generate the substances, energy, and redox forces required for their rapid proliferation. The proliferation of pancreatic ductal ...
By CD BioGlyco.
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Single-Cell RNA Sequencing in Drug Discovery and Development
What Are the Advantages of Single-Cell RNA Sequencing? One of the significant advantages of scRNA-seq in drug discovery is its impact on target identification. By allowing researchers to dissect complex tissues and diseases at the cellular level, scRNA-seq enables the identification of distinct cell subtypes and their gene expression profiles. This information can lead to a better understanding ...
By CD Genomics
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