Dna Strand Articles & Analysis
24 articles found
Understanding Adenovirus and Its Applications Adenoviruses are non-enveloped, double-stranded DNA viruses. Their ability to infect a wide range of host cells makes them ideal candidates for gene transfer. ...
Overview of dsRNA dsRNA molecules consist of two complementary strands that form a helical structure, resembling the double helix of DNA. ...
Reverse Transcription: The 3' cleavage products, now attached to the 5' adaptor, are reverse transcribed into complementary DNA (cDNA). Amplification: The cDNA is amplified through polymerase chain reaction (PCR). ...
Components of NGS Library Preparation Kits NGS Library Preparation Kits are designed to facilitate various stages of the library preparation process, including: Fragmentation: Enzymatic or mechanical methods are used to break down long strands of DNA or RNA into smaller, manageable pieces. This step is crucial for ensuring that the fragments can be effectively ...
Guide RNA alters its sequence for different target sites, while Cas9 functions as a nuclease cutting double strand DNA. At the presence of template, homology directed repair pathway is activated for reconstructing the target site. ...
There are unlimited uses for point mutation cell lines at pre-defined loci, including functional assays, drug screening, FACS screening of membrane proteins, gene expression studies, gene therapy and antibody immunization boosting research. The RNA-guided DNA endonuclease Cas9 associates with a synthetic single guide RNA (gRNA) and cleaves double-stranded ...
Monkeypox virus is an enveloped double-stranded DNA virus. For clinical diagnosis, polymerase chain reaction (PCR) is the preferred laboratory test due to its high accuracy and sensitivity. ...
Fluorescent Microspheres for Nucleic Acid Detection Microspheres encoded with up-converting luminescent materials of different colors can be used to bind different reporter labels, and when bound to single-stranded DNA, the fluorescence intensity will be different. A scientist used a similar method to wrap up-converting luminescent nanoparticles inside ...
FHV-1 Genome Structure and Encoded Glycoproteins FHV-1 is a double-stranded DNA virus with an envelope. The full genome sequence is 126-134 kbp, and the GC content in the base is about 50%. ...
Epstein-Barr Virus (EBV) is a large enveloped virus of double-stranded DNA among the eight known herpesviruses. After EBV infection of cells, it accumulates in the nucleus, and there are two life cycles, with alternating phases of latency and lysis. ...
The development of DNA sequencing technology has made it possible for human beings to explore the mysteries of themselves and other beings, while the advent of the genomics era has put higher demands on sequencing technologies. ...
An important part of CRISPR/Cas9 technology is a nuclease called Cas9, which cuts double-stranded DNA to mediate gene editing. Ideally, Cas9 should be expressed when the gene editing task needs to be completed and inactivated after the gene editing task is completed. ...
Hind II can process DNA precisely, and they also found that methylase can protect the original DNA of bacteria from Hind II's attack by methylation modification. ...
The latter binds to minor grooves in DNA and undergoes Bergman cyclization, producing a highly reactive double radical that induces sequence-selective DNA double-stranded cleavage. ...
Through a multistage biochemical process, these components copy the guide RNA into DNA that ultimately replaces the targeted genome sequence. Importantly, both base and prime editing cut only a single DNA strand, a safer and less disruptive process for cells. ...
Next-Generation Sequencing or NGS has provided scientists alternative approaches in understanding DNA. The technology has come a long way from the simple sequencing technique developed by Sanger in 1977 and continuous improvements are being done to better cater to the needs of researchers. ...
Advances in DNA sequencing technology have made it possible to extensively analyze the breast tumor genome and construct a catalog of gene mutations that may initiate or drive tumor progression. ...
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. These primer sets allow the detection of nuclease Cas9, Nickase Cas9 and double mutant Cas9 at the messenger level. The primer sets are compatible ...
Each makes various trade-offs with respect to these characteristics. Lentiviruses are single-stranded RNA viruses originally derived from natural retroviruses. When used for gene therapy, lentiviruses have the advantage of a large package capacity (~9 kilobases) but present challenges related to genome integration [2]. Adenoviruses are double-stranded ...
What distinguishes AAV from many other viruses is 1) its small genome size and 2) its inability to replicate itself in the absence of a helper virus, hence why the third step, “replicate exponentially and destroy the cell” is missing. As a single-stranded DNA virus, AAV’s genome comprises 4.7 kb of linear DNA. This short ...