Elisa Articles & Analysis: Older
39 articles found
Therefore, it is very important to conduct detailed analysis of the interaction between recombinant protein drugs and FcyR.The analysis of the interaction between recombinant protein drugs and FcyR is mainly carried out through experimental methods such as enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). In ELISA experiments, we can ...
Enzyme-Linked Immunosorbent Assay (ELISA)Using a specific antibody to recognize a specific type of collagen, the content of collagen can be quantitatively analyzed through enzyme-labelled immune reaction and color development.2. ...
These methods are usually simple and low-cost, but their sensitivity and specificity may not be as good as more advanced analytical methods.Enzyme-Linked Immunosorbent Assay (ELISA)ELISA is an immunological method that can be used to detect and quantify specific proteins, including collagen. ...
Enzyme-Linked Immunosorbent Assay (ELISA)This uses phosphorylation-specific antibodies to quantitatively analyze phosphorylation levels in protein samples.Applications1. ...
Proteins from these host cells can mix with the target product, so it is necessary to identify and quantify them through HCP residual detection.Enzyme-Linked Immunosorbent Assay (ELISA)This is a commonly used method that detects HCPs by using antibodies against host cell proteins. Although ELISA is very sensitive, its specificity may be limited because not all ...
ELISAEnzyme-linked immunosorbent assay (ELISA) is the standard method for HCP detection because it provides quick, quantitative results. ...
Immunological MethodsUsing specific antibodies (such as antibodies against specific glycated collagen) for ELISA (enzyme-linked immunosorbent assay) or Western Blot can detect and quantify specific glycated collagen.4. ...
This involves using specific antibodies against acetylation sites, followed by detection with a fluorescently tagged secondary antibody.Enzyme-Linked Immunosorbent Assay (ELISA)If it is necessary to quantitatively analyze the levels of acetylated proteins, the ELISA method can be used.When choosing a method, it is necessary to consider the type of information ...
To find antibodies that can be used as therapeutic antibodies or effectively applied in ELISA, flow cytometry, and blocking assays, we urgently need to design a peptide that can accurately mimic the structure of natural proteins.In the design process, considerations need to be made for the known structure, predicted structure of the target antigen, and practical applications. ...
Linear epitopes are suitable for use in protein blotting (Western Blot) and paraffin-embedded immunohistochemistry experiments, and native epitopes are more suitable for immunoprecipitation (IP), cryosection immunohistology, flow cytometry, and enzyme-linked immunosorbent assay (ELISA). In addition, those antibodies that can identify formalin-resistant epitopes and are used for ...
Vaccine ResponseAfter vaccination, by measuring the antibody levels against vaccine components, an individual's immune response or level of immune protection can be assessed.Drug antibody testing usually requires the collection of blood samples from the test subject, which are then analyzed in the laboratory using testing methods such as enzyme-linked immunosorbent assay (ELISA), ...
This is due to collagen molecules forming an extensive network through different types of cross-linking, making the collagen molecules insoluble and difficult to extract.At present, several types of analysis methods are available for collagen analysis:ELISA of Collagen Specific Propeptide DomainsThis method uses enzyme-linked immunosorbent assay (ELISA) to detect the precursor ...
Specific Staining Method(1) Dyes like Sirius Red or Masson's Trichrome can be used to specifically color collagen.(2) On tissue sections, the stained areas can be quantified using image analysis software to estimate the collagen content.3. ELISA (Enzyme-Linked Immunosorbent Assay)(1) ELISA can use anti-collagen antibodies to quantitatively detect the collagen ...
Experimental VerificationExperimental methods (such as ELISA, flow cytometry, etc.) are used to verify the results of sequence analysis, as well as the impact of constant region changes on antibody function.Analysis of Antibody Constant Region ...
Binding Activity Testing(1) Enzyme-Linked Immunosorbent Assay (ELISA)Uses the target antigen coated on microtiter plates to test the binding ability of the antibody drug.(2) Surface Plasmon Resonance (SPR)Can detect the binding between the antibody and the target molecule in real-time, and determine the rate constants for binding and dissociation.2. ...
Direct Enzyme-Linked Immunosorbent Assay (ELISA) (1) This is a commonly used method to measure the content of specific proteins. ...
It combines the characteristics of ELISA and LC-MS/ MS. First, affinity enrichment of antibodies is achieved by capturing reagents (such as protein A, recombinant antigen, anti-ID antibody, etc.). ...
Advantages of Luminex Technology Traditional cytokine assays, such as ELISA, have limitations that make them less suitable for the demands of oncology. ...
Advantages of Luminex Technology Traditional cytokine assays, such as ELISA, have limitations that make them less suitable for the demands of oncology. ...
This test exhibited a correlation of r=0.98 with a high-sensitivity commercial ELISA assay and a correlation of r=0.90 between matched serum and fingerstick ...