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Elisa Articles & Analysis
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l Validation: Products validated for ELISA, flow cytometry, or ligand-binding kinetics provide higher confidence in reproducibility. ...
These experiments are essential for dissecting the cross-talk between mineralization control and TGF-β superfamily pathways in tissue development and disease. 4. Antibody Development and ELISA Standards Purified recombinant MGP serves as an antigen for monoclonal or polyclonal antibody production. It is also employed as a standard in ELISA kits designed to ...
Antibody Development and Diagnostic Tools Recombinant ELANE serves as an immunogen for the generation of monoclonal antibodies. It is also used in ELISA platforms to quantify ELANE levels in clinical samples—useful for monitoring inflammation in pulmonary and systemic diseases. ...
Methodologies in Cross-Reactivity Studies Various methodologies are employed in tissue cross-reactivity studies. These include: ELISA (Enzyme-Linked Immunosorbent Assay): This is widely used to assess antibody binding to specific antigens present in different tissues. ...
Characterization After selection, characterized clones are further analyzed for their binding affinity, specificity, and stability. Techniques such as ELISA, surface plasmon resonance, and Western blotting are commonly used to evaluate these parameters. 5. ...
This customization can include the selection of specific cytokines, the type of sample (serum, plasma, tissue homogenates, etc.), and the detection method (ELISA, multiplex assays, etc.). Key Features of Customized Rabbit Cytokine Assays Species-Specific Reagents: These assays use antibodies and other reagents specifically developed for rabbit cytokines, ensuring high ...
These beads can simultaneously capture multiple target analytes from one sample — such as cytokines or growth factors — while a detection system measures the interaction through fluorescence readouts. Compared to traditional ELISA assays, this technology maximizes efficiency, saves resources, and provides a broader data set from minimal sample volumes. ...
Given the complexity of the immune system, researchers often need to measure multiple cytokines simultaneously to capture a comprehensive picture of immune activity. Traditional methods, such as ELISA, are limited in throughput and require large sample volumes, making them less suitable for rare or precious samples. ...
Therefore, it is very important to conduct detailed analysis of the interaction between recombinant protein drugs and FcyR.The analysis of the interaction between recombinant protein drugs and FcyR is mainly carried out through experimental methods such as enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). In ELISA experiments, we can ...
Enzyme-Linked Immunosorbent Assay (ELISA)Using a specific antibody to recognize a specific type of collagen, the content of collagen can be quantitatively analyzed through enzyme-labelled immune reaction and color development.2. ...
These methods are usually simple and low-cost, but their sensitivity and specificity may not be as good as more advanced analytical methods.Enzyme-Linked Immunosorbent Assay (ELISA)ELISA is an immunological method that can be used to detect and quantify specific proteins, including collagen. ...
Enzyme-Linked Immunosorbent Assay (ELISA)This uses phosphorylation-specific antibodies to quantitatively analyze phosphorylation levels in protein samples.Applications1. ...
ELISAEnzyme-linked immunosorbent assay (ELISA) is the standard method for HCP detection because it provides quick, quantitative results. ...
Proteins from these host cells can mix with the target product, so it is necessary to identify and quantify them through HCP residual detection.Enzyme-Linked Immunosorbent Assay (ELISA)This is a commonly used method that detects HCPs by using antibodies against host cell proteins. Although ELISA is very sensitive, its specificity may be limited because not all ...
Immunological MethodsUsing specific antibodies (such as antibodies against specific glycated collagen) for ELISA (enzyme-linked immunosorbent assay) or Western Blot can detect and quantify specific glycated collagen.4. ...
This involves using specific antibodies against acetylation sites, followed by detection with a fluorescently tagged secondary antibody.Enzyme-Linked Immunosorbent Assay (ELISA)If it is necessary to quantitatively analyze the levels of acetylated proteins, the ELISA method can be used.When choosing a method, it is necessary to consider the type of information ...
To find antibodies that can be used as therapeutic antibodies or effectively applied in ELISA, flow cytometry, and blocking assays, we urgently need to design a peptide that can accurately mimic the structure of natural proteins.In the design process, considerations need to be made for the known structure, predicted structure of the target antigen, and practical applications. ...
Linear epitopes are suitable for use in protein blotting (Western Blot) and paraffin-embedded immunohistochemistry experiments, and native epitopes are more suitable for immunoprecipitation (IP), cryosection immunohistology, flow cytometry, and enzyme-linked immunosorbent assay (ELISA). In addition, those antibodies that can identify formalin-resistant epitopes and are used for ...
Vaccine ResponseAfter vaccination, by measuring the antibody levels against vaccine components, an individual's immune response or level of immune protection can be assessed.Drug antibody testing usually requires the collection of blood samples from the test subject, which are then analyzed in the laboratory using testing methods such as enzyme-linked immunosorbent assay (ELISA), ...
Specific Staining Method(1) Dyes like Sirius Red or Masson's Trichrome can be used to specifically color collagen.(2) On tissue sections, the stained areas can be quantified using image analysis software to estimate the collagen content.3. ELISA (Enzyme-Linked Immunosorbent Assay)(1) ELISA can use anti-collagen antibodies to quantitatively detect the collagen ...