Human Waste Into Disease Articles & Analysis
75 articles found
In the bustling city of the cell, proteins are the tireless workers. They build structures, carry messages, and catalyze the chemical reactions essential for life. But a protein's job isn't static. Its function, location, and even its lifespan are tightly controlled by a series of modifications. Among the most versatile and critical of these is Protein Ubiquitination, a process that acts like a ...
In recent years, the field of gene editing has experienced groundbreaking advancements, with CRISPR/Cas9 emerging as a powerful technology for precise genetic manipulation. Among various delivery systems, lentiviruses have garnered significant attention due to their ability to introduce genetic material into both dividing and non-dividing cells, making them an ideal vehicle for gene therapy ...
Active Pharmaceutical Ingredients (APIs) form the foundation of modern pharmaceuticals, serving as the biologically active components responsible for the therapeutic effects of medications. These crucial compounds undergo rigorous research, development, and manufacturing processes before being integrated into final pharmaceutical formulations. The pharmaceutical landscape encompasses a diverse ...
In the realm of biomedical research, the study of cytokines and chemokines plays a pivotal role in understanding the immune system and its responses to various stimuli. For researchers working with rat models, the Rat Cytokine and Chemokine Panel offers an invaluable set of tools to investigate these complex signaling molecules. In this article, we will explore what cytokines and chemokines are, ...
Preclinical drug discovery represents a critical phase in the development of new therapeutics. It is the process that bridges the gap between basic research and clinical testing, allowing for the evaluation of potential drug candidates in biological systems before they are tested in humans. Central to this phase is the use of appropriate disease models, which are essential for understanding the ...
The quest for effective therapeutics begins long before a drug reaches clinical trials. One of the cornerstones of this process is drug screening in animal models, which plays a pivotal role in evaluating the safety and efficacy of potential treatments. Animal models offer researchers valuable insights that cannot be obtained through in vitro studies alone, bridging the gap between laboratory ...
The cell cycle is an essential biological process that enables cells to grow, duplicate their DNA, and divide into daughter cells. This cycle is carefully regulated by proteins to ensure accuracy in cell replication. Disruptions in these controls can lead to uncontrolled cell division, a hallmark of cancer and other diseases. Cell cycle progression is orchestrated by key proteins, including ...
In the fast-evolving landscape of biotechnology and systems biology, understanding the dynamic processes that sustain life at a cellular level is crucial for advancing research and therapeutic applications. One essential approach to studying cellular metabolism is Metabolic Flux Analysis (MFA), a powerful tool that reveals the intracellular activities and metabolic dynamics of living systems. ...
Non-alcoholic fatty liver disease (NAFLD) has emerged as a significant public health concern, associated with obesity, insulin resistance, and a spectrum of metabolic disorders. Understanding this condition is crucial for effective intervention and treatment. Preclinical models are indispensable for advancing our knowledge of NAFLD, as they provide the necessary platforms to study its ...
Recombinant protein drugs refer to protein products that originate from animals and plants and are developed through biotechnology research. These protein drugs exhibit certain biological activity and can prevent, diagnose, and treat diseases in humans, animals, and plants. Compared with small molecular drugs, recombinant protein drugs offer advantages such as high activity, high specificity, and ...
Knockin cell line generation represents a pivotal advancement in genetic engineering, providing vital tools for biological research and therapeutic development. This process involves the integration of specific DNA sequences into precise loci within the genome, allowing scientists to explore gene function, model diseases, and develop new treatments. Understanding Knockin Cell Line Generation At ...
Cell culture is one of the major technologies in the life sciences. It is a general term used to remove cells, tissues or organs from animals or plants and place them in an artificial environment conducive to their survival and/or proliferation. The basic environmental requirements for optimal cell growth are: controlled temperature, substrate for cell attachment, appropriate growth medium and ...
Recombinant protein drugs refer to protein products derived from animals or plants and developed through biotechnology research. These proteins possess certain biological activities and can prevent, treat, and diagnose diseases in humans, animals, and plants. Compared to small molecule drugs, protein drugs have advantages such as high activity, high specificity, and low toxicity, making them ...
What is Gene Knockout Technology?Gene knockout (Gene Knockout) is a molecular biology technique that allows researchers to block or shut off the function of a specific gene to study its role and importance. This technique is achieved by introducing mutations into the gene, making it impossible for the gene to express properly or produce functional proteins.Applications of Gene Knockout ...
Autophagy is an intracellular system that degrades cytosolic proteins and organelles. Autophagy can be divided into three groups: macroautophagy, microautophagy and chaperon-mediated autophagy (CMA). Macroautophagy is most extensively studied and best known among the three pathways. When macroautophagy is induced, an isolation membrane encloses a portion of cytoplasm, forming a characteristic ...
Recently developed CRISPR/Cas9-based screening methods have great potential for revealing the mechanisms and treatment strategies of human diseases, but there are still some biological studies that are not suitable for CRISPR knockout screening. CRISPRa and CRISPRi screening opens up many new possibilities. CRISPR/Cas9 PlatformCB provides custom libraries for any set of genes of interest, as well ...
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome ...
Cellular metabolism is the general term for the ordered series of chemical reactions that occur within cells to sustain life. These reactive processes allow organisms to grow and reproduce, maintain their structure, and respond to the external environment. Metabolism is usually divided into two categories: catabolism, which breaks down large molecules for energy (e.g., cellular respiration), ...
Structure of Matrix Metalloproteinase (MMP) Family In vertebrates, the MMP family consists of 28 members, at least 23 of which are expressed in human tissues, 14 of which are expressed in the vascular system. MMPs are usually classified into collagenases, gelatinases, stromelysins, matrilysins, membrane-type (MT)-MMPs, and other MMPs based on their substrates and the organization of their ...
Gene regulation is a complex and tightly controlled process that plays a crucial role in various biological functions, including cell growth, differentiation, and development. CRISPR technology has revolutionized the field of genetics and molecular biology by enabling precise editing of the genome. In recent years, CRISPR activation (CRISPRa) has emerged as a powerful tool for upregulating gene ...