In Fluorescence In Situ Hybridization Articles & Analysis
13 articles found
One of the advanced techniques used to analyze CTCs is Fluorescence In Situ Hybridization (FISH). FISH is a molecular cytogenetic technique that allows for the visualization and localization of specific DNA sequences on chromosomes. It involves using fluorescent probes that bind to specific parts of the genome. This method is ...
Fluorescence In Situ Hybridization (FISH) and Immunohistochemistry (IHC) are two powerful techniques widely used in biological and medical research. ...
PNA monomers form the building blocks of these chains and have found widespread use in the medical, scientific, and agricultural realms because of their exceptional hybridization properties. By binding more strongly and specifically to their complementary DNA or RNA sequences than the native molecules, PNAs can inhibit replication, transcription, or translation processes, which ...
Usually interpretation of HER2 negative cases which has no membrane staining or has faint and weak incomplete cells and also positive cases (3+) that consist of strong and complete cell are easy but cells with moderate and weak HER2 staining (2+ or equivocal) require further analysis with fluorescence in situ hybridization (FISH) technique which ...
Fluorescence in situ hybridization (FISH) approach is constituted of a pair of complementary techniques for precisely detecting gene amplification and over-expression which are regarded as signs of cancer in patients. ...
Fluorescence in situ hybridization (FISH) is a technique that prepares acceptable results for molecular imaging biomarkers to precisely and dependably detect and diagnose disorders which are sign of cancers. ...
Culture-independent methods, including DNA hybridization (e.g., microarray and fluorescent in situ hybridization), DNA cloning, and PCR, have been employed to detect individual members and/or functional genes in microbial communities in an attempt to overcome this fundamental issue. ...
It’s not often you lovingly hug a 450-pound piece of equipment, but if it makes your work life easier you show your appreciation. The CDS-5 helps lab technologists create consistent and reliable environments for their work regardless of the day-to-day variables, like the weather. Imagine the weather outside impacting your office work indoors. Sounds inconvenient, right? That used to be a ...
For image acquisition we used optical coherence tomography to non-destructively visualize the three dimensional structure of entire internal cavities and fluorescent in situ hybridization of carrier cryosections to visualize the spatial distribution of ammonia oxidizing bacteria and nitrite oxidizing relative to the overall biofilm structure. ...
Although with the emergence of molecular methods such as Terminal Restriction Fragment Length Polymorphism (TRFLP), Automated rRNA Intergenic Spacer Analysis (ARISA), Random Amplified Polymorphic DNA (RAPD), Denaturing Gradient Gel Electrophoresis, Single Strand Conformation Polymorphism (SSCP), microautoradiography (MAR) and Fluorescent in situ ...
Phylogenetic characterization of this microbial community was done via 16S rRNA gene clone library and gradient gel electrophoresis analyses. Fluorescence in situ hybridization was used to quantify populations of 'Dehalococcoides' and Archaea and to examine the colocalization of these two groups within culture bioflocs. ...
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is a method that is widely used to detect and quantify microorganisms in environmental samples and medical specimens by fluorescence microscopy. Difficulties with FISH arise if the rRNA content of the probe target organisms is low, causing dim ...
A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with ...