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Intestinal Stem Cell Articles & Analysis: Older

21 articles found

In Vitro Permeability Assay Kits: A Comprehensive Overview

In Vitro Permeability Assay Kits: A Comprehensive Overview

In vitro permeability assay kits play a crucial role in the field of pharmaceutical development, particularly when evaluating the absorption characteristics of drugs. These kits allow scientists and researchers to investigate how compounds permeate biological membranes, which is vital for understanding drug bioavailability. Understanding Permeability Permeability refers to the ability of a ...

ByCreative Bioarray


Glycolysis, Lipid Metabolism, Coenzymes and Cofactors in Cellular Metabolism

Glycolysis, Lipid Metabolism, Coenzymes and Cofactors in Cellular Metabolism

Cellular metabolism is the general term for the ordered series of chemical reactions that occur within cells to sustain life. These reactive processes allow organisms to grow and reproduce, maintain their structure, and respond to the external environment. Metabolism is usually divided into two categories: catabolism, which breaks down large molecules for energy (e.g., cellular respiration), ...

ByCreative Proteomics


Regulation of Lipid Metabolism in the Intestinal Flora

Regulation of Lipid Metabolism in the Intestinal Flora

Trillions of microorganisms live in the gastrointestinal tract, far outnumbering the body's own cells. The intestinal flora is involved in many physiological and pathological processes in the host, including digestion and absorption of food, metabolism of certain nutrients and pharmaceutical compounds, development of host immunity, intestinal inflammatory states, and more. Dysbiosis of the ...

ByCreative Proteomics


Bile Acid: A Comprehensive Exploration of Function and Metabolism

Bile Acid: A Comprehensive Exploration of Function and Metabolism

In the realm of biological sciences, bile acids stand as pivotal molecules, playing a multifaceted role in various physiological processes within organisms. Bile acids are specialized molecules synthesized from cholesterol within the liver. These amphipathic compounds possess a hydrophilic region (carboxylic acid moiety) and a hydrophobic region (steroid backbone). Their distinctive structure ...

ByCreative Proteomics


Applications of Diamine Oxidase

Applications of Diamine Oxidase

Diamine oxidase (DAO) is an intracellular enzyme that catalyzes diamine in mucosal or ciliated epithelial cells of the mammalian small intestine. It protects the intestinal mucosa by regulating intracellular ion homeostasis, influencing conduction pathways, and promoting cellular repair. Serum DAO is elevated in small intestinal mucosal barrier failure or in intestinal mucosal cell necrosis; ...

ByCreative Enzymes


A Beginner’s Guide to Short Bowel Syndrome

A Beginner’s Guide to Short Bowel Syndrome

Created for parents, by parents. No parent expects the news. So when you receive the news of a Short Bowel Syndrome diagnosis, it’s only fair that it’s a little overwhelming. Your picture of what the future of your family looks like begins to shift, and you have to shift with it. Let’s start off by saying, you’re not alone. There are parents, caregivers and family ...

ByEclipse Regenesis


The Importance of Moderate Supplementation of Intestinal Lactase

The Importance of Moderate Supplementation of Intestinal Lactase

Lactase is a disaccharidase present on the surface of mammalian small intestinal mucosal microvilli, and many beneficial intestinal bacteria have the ability to produce lactase. Lactase deficiency is present in most of the world's population, affecting nearly 2/3 of the world's population. Polylactose is composed of glucose and galactose, these two monosaccharides are easily absorbed by the ...

ByCreative Enzymes


Sources and Functions of Short-Chain Fatty Acids

Sources and Functions of Short-Chain Fatty Acids

When we talk about intestinal bacteria or dietary fiber, we often hear about "short-chain fatty acids". So what exactly are short-chain fatty acids? Based on the number of carbon atoms in the carbon chain, fatty acids with less than 6 carbon atoms are commonly referred to as short-chain fatty acids (SCFAs), those with 6 to 12 carbon atoms are called medium-chain fatty acids, and those with 12 or ...

ByCreative Proteomics


Human Intestinal Ecosystem Analysis

Human Intestinal Ecosystem Analysis

The gut is a complex ecosystem that contains an extremely large community of microbes known as the gut microbiome. The gut microbiome is made up of bacteria, archaea, viruses, fungi, and other eukaryotic microorganisms, including yeasts, flagellates, ciliates, and protozoa. The gut microbiota of a healthy individual consists of six phyla (Firmicutes, Bacteroidetes, Proteobacteria, ...

ByCreative Proteomics


The Power Of RepliGut in Vitro Intestinal Stem Cell Models

The Power Of RepliGut in Vitro Intestinal Stem Cell Models

This single cell type model allows for investigation into the physiology of intestinal epithelial cells as proliferative stem or differentiated cell types, depending on the researchers’ interest. ...

ByAltis Biosystems


Anti Helicobacter Pylori Formula Shows Initial Results

Anti Helicobacter Pylori Formula Shows Initial Results

Helicobacter pylori (Hp) is closely related to the occurrence and development of gastric cancer, peptic ulcer, Hp-related dyspepsia and other diseases. Studies have found that Hp can cause gastric microbial disorders, change the microbial diversity and community structure in the stomach, and cause precancerous lesions. The public health community has made great efforts to eradicate Hp for a long ...

ByCreative Enzymes


Accurately Recapitulating Environmental Niches in  Vitro Models of the Intestine

Accurately Recapitulating Environmental Niches in Vitro Models of the Intestine

Advanced microphysiological systems can replicate aspects of intestinal complexity, such as epithelium self-renewal by stem cells in in vitro crypts or the interactions of microbes and intestinal epithelium mediated by a mucus layer. ...

ByAltis Biosystems


Recapitulating the Multifaceted Inflammatory Environment of the Intestine In Vitro

Recapitulating the Multifaceted Inflammatory Environment of the Intestine In Vitro

From each donor, intestinal epithelial stem cells are isolated from each region of the small intestine and colon (duodenum, jejunum, ileum, ascending colon, transverse colon, and descending colon) for use on our RepliGut® in vitro platform. ...

ByAltis Biosystems


How Does Altis Biosystems Envision the Future of the Pharma/Biopharma Industry?

How Does Altis Biosystems Envision the Future of the Pharma/Biopharma Industry?

Human intestinal stem cells, by contrast, are already epigenetically modified to limit them to a range of differentiated types but are then free to form the appropriate diversity of intestinal epithelial cell types. As such, the Altis system uses primary human stem cells to ...

ByAltis Biosystems


Enhancing Human Gut Tissue Models through Gene Editing

Enhancing Human Gut Tissue Models through Gene Editing

The RepliGut® platform produces a polarized monolayer of human intestinal stem and differentiated cells — either of the large or small intestine — that can be used for modeling diseases or screening compounds for effects on the intestine. ...

ByAltis Biosystems


Increasing the Efficiency of Drug Development with Preclinical Testing Using Human Intestinal Stem Cells

Increasing the Efficiency of Drug Development with Preclinical Testing Using Human Intestinal Stem Cells

Based on human intestinal stem cells, RepliGut® eliminates the issues associated with the use of colon cancer cells. ...

ByAltis Biosystems


Evaluating Microbiome Therapies with Intestinal Cell Models

Evaluating Microbiome Therapies with Intestinal Cell Models

Intestinal and Colonic Stem Cells and Disease Stem cells are implicated in a variety of disease process. ...

ByAltis Biosystems


Dissolved Hydrogen Application

Dissolved Hydrogen Application

Hydrogen molecule is the smallest molecule in nature. It is extremely penetrating and extremely volatile. It can freely enter and exit the body cells, remove aging and toxic free radicals. After drinking, it enters human body tissues and diffuses to act on all organ line particles and nucleus. Absorbed by the body. Realize the balance of the environment in the body and initiate the mechanism of ...

ByShanghai Edon Mechanical & Electrical Equipment Co., Ltd.


Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells

Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells

Abstract We previously generated 32 rotavirus-specific (RV-specific) recombinant monoclonal antibodies (mAbs) derived from B cells isolated from human intestinal resections. Twenty-four of these mAbs were specific for the VP8* fragment of RV VP4, and most (20 of 24) were non-neutralizing when tested in the conventional MA104 cell–based assay. We reexamined the ability of these mAbs to ...

ByJohnson Controls, Inc.


Defining cell culture conditions to improve human norovirus infectivity assays

Defining cell culture conditions to improve human norovirus infectivity assays

Significant difficulties remain for determining whether human noroviruses (hNoV) recovered from water, food, and environmental samples are infectious. Three-dimensional (3-D) tissue culture of human intestinal cells has shown promise in developing an infectivity assay, but reproducibility, even within a single laboratory, remains problematic. From the literature and our observations, we ...

ByIWA Publishing

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