Lentivirus Articles & Analysis
7 articles found
Its simplicity, efficiency, and versatility have made CRISPR/Cas9 a popular choice for various biological research purposes, ranging from functional genomics to therapeutic interventions. Advantages of Lentivirus as a Delivery System Lentiviruses, a subclass of retroviruses, play a crucial role in the delivery of CRISPR/Cas9 components. ...
Features Improved biosensor expression efficiency with lentiviral transduction Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells Non-disruptive towards cellular function Live cell time-lapse imaging of lentivirally-transduced cells Enables visualization under different cell/disease states in live cell and in vitro analysis ...
Our miRNA overexpression stable cell lines are constructed by lentivirus transduction or non-viral plasmid transfection of vectors optimized by our scientists to enable expression and maturation of miRNAs inside cells. ...
What are Lentiviral Particles? Derived from the Lentivirus genus, lentiviral particles are a type of retrovirus renowned for their ability to integrate their genetic material into the host cell's genome. ...
Development of Lentiviral Vectors A variety of strategies are used to improve the expression and transduction efficiency of lentivirus vectors and achieve tissue or cell type specificity to help researchers design and develop efficient and safe lentivirus vectors. ...
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. These primer sets allow the detection of nuclease Cas9, Nickase Cas9 and double mutant Cas9 at the messenger level. The primer sets are compatible ...
However, they tend to trigger strong immune responses in humans, which limits their applicability for gene therapy applications, particularly in immunocompromised patients [1]. Compared to adenovirus and lentivirus, AAV offers a low immune profile, relative safety, long-lasting expression in non-dividing cells, and a long scientific and clinical history. ...