Nucleic Acid Extraction Articles & Analysis
6 articles found
Wet lab endeavors encompass sample pretreatment, nucleic acid extraction, library construction, and ultimately, sequencing. ...
Cell lysates were prepared as described in the “Preparation of cell lysates and WES™ analysis” section for WES™ analysis. 2.4 Virus titer determination The viral titers of IAV and SARS-CoV-2 viruses were determined using a real-time one-step multiplex RT-qPCR method. Briefly, viral nucleic acid extraction was performed ...
By designing and synthesizing specific probes targeting the target region (human genome or microbial specific region, etc.), hybridizing with the target genomic library DNA containing the target region in the sample, capturing and then performing high-throughput sequencing using a mainstream sequencing platform. The sample nucleic acid is ...
The following article demonstrates how the coronavirus detection workflow in wastewater – from sampling, enrichment, and nucleic acid extraction to real-time PCR detection - can be efficiently implemented and how it can be used as an effective tool for COVID-19 management. ...
Overview of the SARS-CoV-2 (Coronavirus) Detection Process In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 105,000,000 confirmed cases by beginning of February 2021 the World Health Organization declared the outbreak a public health emergency of international ...
Viruses were recovered from the samples using a glass wool adsorption-elution method and then further concentrated using polyethylene glycol/sodium chloride precipitation. After automated nucleic acid extraction, samples were analysed for HAV by real-time reverse-transcriptase polymerase chain reaction. ...