Protein Acetylation And Ubiquitination Interactions Control Mcl1 Protein Articles & Analysis
23 articles found
What Is PROTAC Technology? Proteolytic targeting chimera (PROTAC) is a new type of drug design technology that works by inducing the degradation of target proteins. Unlike conventional small molecules that typically inhibit protein function, PROTACs facilitate the ubiquitination and subsequent degradation of specific proteins. This bifunctional molecule consists of two distinct elements: a ...
Ubiquitination is a post-translational modification process that involves various biological processes, including signal transduction, cell cycle regulation, cell stress responses, and cell apoptosis. It plays a crucial role in maintaining protein homeostasis, regulating various biological reactions in cells, and ensuring the normal physiological functions of organisms. Immunoprecipitation is a ...
Ubiquitination is a widely present method of protein modification. It manages its designation, activity, stability, and interaction by marking proteins, playing a crucial role in the regulation of cellular functions and biological processes. Therefore, measuring the ubiquitination level of a protein is significant for understanding its function and regulation mechanism. This article mainly ...
Protein Ubiquitination is a crucial cellular biological process. It involves the binding of Ubiquitin protein to the target protein, thereby regulating the degradation, positioning, activation, etc., of the target protein. To detect protein ubiquitination, methods such as Immunoprecipitation (IP) combined with Western blot, mass spectrometry analysis, etc., are commonly used. Here are the typical ...
Western blot can accurately detect the presence and expression of specific proteins in samples, and it can also be used to detect protein post-translational modifications, such as phosphorylation, ubiquitination, etc. The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not ...
Commonly Used Ubiquitinated Protein Enrichment Methods1. Ubiquitin Antibody EnrichmentThis is one of the most common methods for enriching ubiquitinated proteins. Researchers use high-affinity ubiquitin antibodies to bind to ubiquitinated proteins, then use methods such as immunoprecipitation or affinity chromatography to bind the ubiquitinated proteins to the antibodies, which can then be used ...
Post-translational modification (PTM) refers to the biochemical process where specific amino acid residues of a protein are chemically modified after its translation. These chemical modifications greatly expand the functional diversity of proteins, enabling them to participate in more cellular physiological processes. The following are some common post-translational modifications of proteins:1. ...
Tau protein is a microtubule-associated protein, mainly present in nerve cells, and participates in the stabilization of microtubules. In certain neurodegenerative diseases, such as Alzheimer's disease (AD), Tau protein may undergo abnormal phosphorylation and aggregation, forming so-called Tau tangles or neurofibrillary tangles.Ubiquitination is a kind of post-translational modification of ...
Co-immunoprecipitation (Co-IP) is an experimental technique used to detect the interaction between a protein and other proteins. When it comes to detecting protein ubiquitination, Co-IP can be used to determine whether the target protein is ubiquitinated, and its interaction with ubiquitin protein.Analysis Workflow1. Sample PreparationCollect cellular or tissue samples, and lyse the cells with ...
In post-translational modification (PTM) of proteins, ubiquitination is considered one of the most critical processes in regulating cellular functions and various diseases. The identification of ubiquitination sites becomes very crucial for understanding the mechanisms of ubiquitination-related biological processes. Experimental and computational methods can be used to identify ubiquitination ...
The ubiquitination site of a protein refers to those specific amino acid residues on the protein molecule that can covalently bind with Ubiquitin. Ubiquitin is a small protein that plays an important regulatory role in cells, particularly in protein degradation and signal transduction. Ubiquitination is a post-translational modification process where ubiquitin molecules are attached to specific ...
Histone modification is an important epigenetic regulatory mechanism, achieved by adding or removing various chemical groups to specific amino acid residues on histones. These modifications can influence the structure and function of chromatin, thereby regulating gene expression. To date, at least nine different types of histone modifications have been discovered. Acetylation, methylation, ...
Ubiquitination detection of proteins is a biochemical technique used to identify and analyze ubiquitination modifications on proteins. Ubiquitination is a post-translational modification process that involves the covalent linkage of the small protein ubiquitin to lysine residues of a target protein, a modification that plays a crucial role in the regulation of protein degradation, signal ...
Post-translational modifications (PTMs) of histones are crucial for protein structure regulation, playing key roles in gene expression regulation, DNA repair, cell signaling, and more. These PTMs of histones can be achieved by adding or removing different chemical groups or proteins, thus affecting chromatin structure and function. Here are some common types of histone PTMs. 1. Acetylation The ...
Proteolysis Targeting Chimeras (PROTACs) are a novel approach for degrading target proteins. Recently, a new split-and-mix PROTAC (SM-PROTAC) system based on liposome self-assembly, known as LipoSM-PROTAC, was reported in the Journal of the American Chemical Society. The LipoSM-PROTAC system exhibits selective degradation of target proteins, with folate serving as a key ligand. In this study, the ...
The concept of proteolytic targeting chimera (PROTAC) was first proposed by Crews and Deshaies laboratory in 2001, the team successfully achieved the degradation of methionyl aminopeptidase 2 (MetAp-2) by PROTAC method. Since then, PROTACs targeting androgen receptor (AR) and estrogen receptor (ER) have appeared one after another. In 2004, a PROTAC molecule that recruited the tumor suppressor ...
Longlong Si's research group from the Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, published a research titled "Generation of a live attenuated influenza A vaccine by proteolysis targeting" in Nature Biotechnology. Using influenza virus as a model virus, the team established the technology of protein degradation targeting virus as an ...
Diabetes affects more than 400 million people worldwide and is a huge public health burden. Obesity is a major risk factor for diabetes and leads to insulin resistance that often precedes diabetes. Currently, genome-wide association studies (GWAS) have identified more than 80 diabetes susceptibility loci, and an increasing number of mutations and variants have been identified in monogenic forms ...
Targeted protein degradation (TPD) technology is a new technology that specifically recognizes the target protein and directly degrades the target protein using the inherent protein degradation pathway in the cell. At present, technologies such as PROTACs, molecular glues, degradation tags, lysosome-targeted chimeras, and autophagosome-binding compounds have been developed in the field of TPD, ...
The mode of action of currently developed drugs is mostly to achieve the purpose of disease treatment by inhibiting or enhancing the activity of target proteins. However, these drugs have to bind to the active site of target proteins to work. Among the known drug target proteins, only about 20% of the target proteins contain bindable active site, and 80% of the target proteins are non-druggable ...