Real Time Quantitative Pcr Articles & Analysis
37 articles found
The recent COVID-19 pandemic again highlighted the urgent need for broad-spectrum antivirals, both for therapeutic use in acute viral infection and for pandemic preparedness in general. The targeting of host cell factors hijacked by viruses during their replication cycle presents one possible strategy for development of broad-spectrum antivirals. By inhibiting the Raf/MEK/ERK signaling pathway, ...
What is Chimeric RNA? Chimeric RNA, also known as fusion RNA, refers to RNA molecules that are formed as a result of the fusion of two or more different RNA transcripts. Chimeric RNA, a unique class of RNA molecules formed through various molecular mechanisms, such as trans-splicing, read-through transcription, and fusion genes. These hybrid transcripts play a significant role in diverse ...
SARS-CoV-2 is a newly discovered human coronavirus group (HCoV), which are single-stranded RNA viruses belonging to the family of Coronaviridae. A range of respiratory diseases have been induced by some of these pathogens in the past. SARS-CoV-1 infected more than 8,000 individuals around the world, for example. Human coronavirus linked to Middle East respiratory syndrome (MERS-CoV) also occurred ...
Introduction to Quantitative Polymerase Chain Reaction A highly delicate technique for the amplification and identification of deoxyribonucleic acid is polymerase chain reaction (PCR). ...
Regulatory agencies worldwide have imposed strict limits on the amount of rcDNA, and various pharmacopoeias have outlined several reliable methods for residual DNA quantification, including threshold methods, hybridization methods, and real-time quantitative PCR. The Potential Risks of Host Cell Residual DNA: Host cell lines ...
While the world continues to endure loss and hardship due to the outbreak of COVID-19, caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), researchers are scrambling to provide test kits that are accurate, fast, sensitive, and widely available, especially as symptoms vary broadly and may not appear for days if at all. To this end, several diagnostic methods have been ...
What is lyophilization? Lyophilization in biotechnology is the process of freeze-drying, removing moisture content from a product at low temperature until it is no longer biologically or chemically active. Microbes, proteins, pharmaceuticals, plasma, and tissues are just a few examples of the many heat-sensitive things that can be preserved with the freeze-drying technique. Lyophilization ...
ByFireGene
Wastewater & COVID-19 Connections When the word wastewater crosses your mind, there is probably also a memory of your Mom’s voice telling you – wash your hands! When humans transitioned into community living, keeping your living space separate from your bathroom space was an important rule to learn. We can thank early scientists who were curious about how diseases spread and ...
A real-time PCR cycler can be a significant investment for a lab, and if you’re responsible for the purchase decision you’ll want to do your due diligence and make a future-proof choice. So where do you begin your search? ...
We have great news to share: we launched Schep SARS-CoV-2 Multi-FAST, a new PCR diagnostic test for the rapid detection of SARS-CoV-2, now in multiplex and in a single step format! Simple detection, by reverse transcription and Real Time PCR. It consists of a single Master Mix, ready to use, containing the enzymes, primers and probes, specific for 3 viral genes: Envelope (E), Nucleocapsid (N) ...
The Argentinian National Administration of Drugs, Food and Medical Technology (ANMAT) approved New kitfor the qualitative detection of the SARS-CoV-2 virus based on Real-Time RT-PCR technology. The Argentinian National Administration of Drugs, Food and Medical Technology (ANMAT) approved Schep SARS-CoV-2 RT-PCR Duo Biocientfica SA, an Argentinian biotechnology company developed this sensitive in ...
In light of the Delta variant, Mu variant, and breakthrough cases in vaccinated individuals, COVID-19 still presents a challenge for travelers. Requirements change quickly and vary by country or region. One of the biggest challenges is making sure travelers can be tested and receive test results quickly enough, since the longer someone has to wait for test results, the longer they have to isolate ...
ByIGeneX
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. These primer sets allow the detection of nuclease Cas9, Nickase Cas9 and double mutant Cas9 at the messenger level. The primer sets are compatible ...
As the world opens up over the coming months and years, there will be countless plane journeys to see loved ones, meet new family members and travel for work and leisure. Many of these travellers will first need to provide evidence of a negative PCR test to show they pose a minimal COVID-19 transmission risk. Time is proving of the essence for testing. With reports of multi-hour queues at ...
The company’s longer-term goal is to develop the platform to provide “multiple points of information from a single test,” says President Tej Patel. Fluxergy, a company that has already made headlines for earning a CE mark for its one-hour COVID-19 RT-PCR test, has expanded its U.S. headquarters after commercializing the test globally. The expansion will enable the company to ...
ByFluxergy
Back in early 2020, amongst the worthy efforts to halt its spread, the COVID-19 pandemic started wreaking havoc on scientific endeavours all over the world. However it did not affect all scientists the same way. While some had their research grind to a halt, others have pivoted or have been redirected to support the new global cause – everything from conducting RT-PCR ...
Mic qpcr cycler and Myra liquid handler have been put through their paces in COVID-19-testing field trials in regional Western Australia and found to be noticeably robust, rapidly deployable and flexible. These results have been outlined in a recent paper, Development, deployment and in-field demonstration of mobile corona virus SARS-CoV-2 Nucleic acid amplification test, published by Paton, ...
SARS-CoV-2 detection workflow in wastewater Monitoring wastewater for SARS-CoV-2 – or Coronavirus as it is more commonly known – can be a valuable data resource for an effective COVID-19 management. However, there are several challenges involved in detecting biological parameters in wastewater. Fortunately, there are also several solutions available to overcome them. Wastewater ...
Overview of the SARS-CoV-2 (Coronavirus) Detection Process In December 2019 the novel SARS-CoV-2 (formerly named 2019-nCoV) was identified in Wuhan, the capital of China’s province Hubei. Based on its rapid spreading with more than 105,000,000 confirmed cases by beginning of February 2021 the World Health Organization declared the outbreak a public health emergency of international ...
Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. ...