Cellomics Technology, LLC products

eSpCas9 (enhanced specificity Cas9) has enhanced specificity compared to wild type Cas9 for CRISPR/Cas9 genome editing. eSpCas9 is able to reduce off-target effects and maintain robust on-target genome editing efficiency, allowing more efficient gene targeting. eSpCas9 (CAG, Neo) lentivirus expresses eSpCas9 under the control of the CAG promoter with neomycin as a selection marker, allowing selecting stable cell clones with neomycin.

CISH (Cytokine Inducible SH2 Containing Protein) contains a SH2 domain and a SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CISH may be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

EZ-Transfx transfection reagent is a highly efficient, easy-to-use transfection reagent for delivery plasmid DNA into eukaryotic cells. EZ-Transfx is a sterile solution of a synthetic polymer in water.

Lentiviral vector-CAG, BSD contains  Multiple Cloning Sites (MCS) downstream of the CAG promoter, and blasticidin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as HEK293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by blasticidin.

Purification and concentration of lentivirus may be a tedious and time-consuming process. ViroGold lentivirus Concentrator provides a simple, efficient, and time-saving method to concentrate and purify lentivirus in a simple spin without ultra-centrifugation and ultrafiltration. Lentiviruses can be concentrated over thousand of folds. Most importantly, the concentrated virus is ultrapure, very easy to dissolve, and ready for both in vitro and in vivo use. After concentration, dissolve the lentivirus in 1xPBS or other buffer, add LentiGuard to prevent lentivirus titer loss during freeze-thaw cycles.

LentiGuard® is a new product developed to preserve functional lentivirus titer during freeze-thaw cycles. Lentivirus titer may drop 50% with just one freeze-thaw cycle without LentiGuard®. Adding LentiGuard® to the lentivirus supernatant or concentrated lentivirus, the functional virus titers are preserved 90% even after six freeze-thaw cycles.

Lentiviral packaging is the most critical step for generating high titer of lentivirus. EZ-LentiPACK is an optimized and ready-to use packaging system for the production of the highest titers of replication-incompetent lentivirus.

LentiMeter™ is a lentivirus titration kit for rapid and accurate estimation of the functional lentivirus titer in less than 10 minutes. LentiMeter™ is a lateral flow immunoassay (LFIA) based titration device, based on p24 detection with sensitive gold-conjugated antibody. Lentiviral supernatant standards functionally titrated at 106 TU/mL are included in the kit. By comparing the intensities of the test lines of the standards and that of unknown sample, the cassette can help accurately estimate lentiviral TU titer, using p24 as a gauge. It can also be used to determine the optimal time when packaging preparation is ready for harvesting.

Lentiviral vector transduction is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells. Lentiviral vector can stably integrate into the host genome, allowing long-term stably, high-level gene expression both in vitro and in vivo. Standardized and optimized lentivirus particle production and strict quality control system leads to high titers of recombinant lentivirus particles.

C2C12 cells have been widely used in myoblast differentiation research. C2C12/Cre-GFP stable cells express Cre recombinase fused with GFP under the control of the CMV promoter. Cre-loxP system has been widely used for gene editing such as deletion, inversion and translocation. This Cre-GFP stable cell line is convenient for using to test your loxP constructs in C2C12 cells.