provitro AG products
Immunohistochemistry
Provitro - Morphological Structure Tissue
The morphological structure of tissue can be depicted with high accuracy by means of histological sections. Staining results depend on numerous factors, including pH of the buffer solution, staining time and method of fixation. Haematoxylin, for example, is used to stain cell and tissue structures, such as cell nuclei, mitochondria, myelin, elastin and collagen fibres. Additional information may be obtained from counterstaining (differential staining), using a dyestuff in high contrast to hematoxylin stain. Counterstaining, using eosin, is a classical approach for which cationic structures are stained (e.g. proteins).
Primary Cell Cultures
Provitro - Human Endothelial Cells
Endothelium is the cover term for the blood-oriented cells in the innermost layer of the vascular wall (intima). Functioning as physiological barrier to tissue, the CD 31- and vWF-positive cells express nitrogen oxide (NO) which plays a role in regulating the tonus of the vascular musculature. Damage to the endothelial cell layer has been assumed to be possibly a causative factor in the development of arteriosclerosis. Endothelium plays an equally important role in inflammatory processes and may be locally activated by various endogenous or microbial substances.
Tissue Microarrays (TMA)
Provitro - Customised Tissue Microarrays (TMA)
Customised TMA include service of tissue microarray manufacturing and slide cutting according to customer’s needs. Additional immunohistochemistry service is available on request.
Provitro - Inflammatory + Autoimmune Tissue Microarrays (TMA)
Cultures based on cell monolayers have the advantage of reducing tissue complexity to a minimum. However, the lack of normal physiological cell-matrix interactions leads the cell metabolism and gene expression towards a dedifferentiated state. Compared to a monolayer, three-dimensional cultures are associated with a higher cell density, and nutrient supply depends on diffusion through the extracellular matrix. This requires more exacting culture conditions which can be achieved using automated medium replacement in a perfusion culture system, thereby guaranteeing a stable concentration of nutrients, stable pH, and reduced risk of contamination.
Provitro - Cardiovascular Tissue Microarrays (TMA)
Cardiovascular TMA include samples from cardiovascular normal tissue and myocardial infarction, myocardial hypertrophy of left heart/right heart and vascular tissue samples including classification of artheriosclerosis according to Stary.