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Kisspeptin-54(human)

MCE China：Kisspeptin-54(human)

Brand：MedChemExpress (MCE)

Cat. No.HY-P1022

CAS：374683-24-6

Synonyms：Metastin(human)

Purity：98.00%

Storage：Sealed storage, away from moisture and light, under nitrogen Powder -80°C 2 years -20°C 1 year *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Kisspeptin-54(human) (Metastin(human)) is an endogenous ligand for kisspeptin receptor (KISS1, GPR54). Kisspeptin-54(human) binds to rat and human GPR54 receptors with Ki values of 1.81 nM and 1.45 nM, respectively. Kisspeptin-54(human) inhibits tumor metastasis and stimulates the secretion of gonadotropin (LH) and testosterone.

In Vitro：Kisspeptin-54(human) (1-100 nM, 4-48 h) stimulates aldosterone synthesis in human adrenal cells (H295R cells and fetal adrenal neocortex cells)[3]. Kisspeptin-54(human) (1-10 μM, 12 h) suppresses migration of human pancreatic cancer cells (PANC-1) [4]. Kisspeptin-54(human) (10-1000 nM, 14-16 h for migration, 24-48 h for invasion) inhibits migration and invasion of human renal cell carcinoma cells (Caki-1 and ACHN) with overexpression of metastireceptor[5].

In Vivo：Kisspeptin-54(human) (0.3-100 nmol/kg, i.p., 30 min before trunk blood and anterior pituitaries are collected) dose-dependently shows activation of the anterior pituitary release of LH in post-pubertal male rats[6]. Kisspeptin-54(human) (0.3-30 nmol/kg, i.p.) produces a dose-dependent effect on c-fos mRNA levels in the anterior pituitary in various ages (PND15, PND30, and PND45) male rats[6]. Kisspeptin-54(human) (0.1-30 nmol/kg, i.p., single) increases serum testosterone in mice, with the same potency as for mouse kisspeptins[7]. Kisspeptin-54(human) (6.7 nmol/rat, s.c.) induces the release of gonadotropin via activation of the hypothalamic GnRH neurons in prepubertal female rats[8].

IC50 & Target：Ki: 1.81 nM (Rat GPR54) and 1.45 nM (Human GPR54)[2] In Vitro Kisspeptin-54(human) (1-100 nM, 4-48 h) stimulates aldosterone synthesis in human adrenal cells (H295R cells and fetal adrenal neocortex cells)[3]. Kisspeptin-54(human) (1-10 μM, 12 h) suppresses migration of human pancreatic cancer cells (PANC-1) [4]. Kisspeptin-54(human) (10-1000 nM, 14-16 h for migration, 24-48 h for invasion) inhibits migration and invasion of human renal cell carcinoma cells (Caki-1 and ACHN) with overexpression of metastireceptor[5]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Kisspeptin-54(human) Related Antibodies Cell Migration Assay [4] Cell Line: PANC-1, AsPC-1

Sequence：Gly-Thr-Ser-Leu-Ser-Pro-Pro-Pro-Glu-Ser-Ser-Gly-Ser-Arg-Gln-Gln-Pro-Gly-Leu-Ser-Ala-Pro-His-Ser-Arg-Gln-Ile-Pro-Ala-Pro-Gln-Gly-Ala-Val-Leu-Val-Gln-Arg-Glu-Lys-Asp-Leu-Pro-Asn-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2

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References：

[1]. M L Gottsch, et al. A Role for Kisspeptins in the Regulation of Gonadotropin Secretion in the Mouse. Endocrinology. 2004 Sep;145(9):4073-7.  [Content Brief]

[2]. M Kotani, et al. The Metastasis Suppressor Gene KiSS-1 Encodes Kisspeptins, the Natural Ligands of the Orphan G Protein-Coupled Receptor GPR54. J Biol Chem. 2001 Sep 14;276(37):34631-6.  [Content Brief]

[3]. Nakamura Y, et al. Metastin stimulates aldosterone synthesis in human adrenal cells. Reprod Sci. 2007 Dec;14(8):836-45.  [Content Brief]

[4]. Masui T, et al. Metastin and its variant forms suppress migration of pancreatic cancer cells. Biochem Biophys Res Commun. 2004 Feb 27;315(1):85-92.  [Content Brief]

[5]. Shoji S, et al. Metastin inhibits migration and invasion of renal cell carcinoma with overexpression of metastin receptor. European urology, 2009, 55(2): 441-451.

[6]. Bentsen AH, et al. Maturation of kisspeptinergic neurons coincides with puberty onset in male rats. Peptides. 2010 Feb;31(2):275-83.  [Content Brief]

[7]. Mikkelsen JD, et al. Comparison of the effects of peripherally administered kisspeptins. Regul Pept. 2009 Jan 8;152(1-3):95-100.  [Content Brief]

[8]. Matsui H, et al. Peripheral administration of metastin induces marked gonadotropin release and ovulation in the rat. Biochem Biophys Res Commun. 2004 Jul 23;320(2):383-8.  [Content Brief]