Aves - Model GFP-1020 - Anti-Green Fluorescent Protein Antibody
From Primary Antibodies
Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).
Details
- Target Green Fluorescent Protein (GFP)
- Target Description Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.
- Format IgY Fraction
- Concentration 10 mg/mL
- Clonality Polyclonal
- Isotype IgY
- Applications ELISA, Flow, ICC, IHC, WB
- Host Species Chicken
- Molecular Weight 27 kDa
- Antigen Recombinant GFP expressed in Escherichia coli
- Antibody Registry ID AB_2307313
- Storage Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.
- Physical State Liquid
- Production Notes Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).
- Buffer Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.
- Dilution Ranges WB: 1:5000-1:10000
- IHC: 1:2000-1:5000
- ICC: 1:2000-1:5000
- Quality Control Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.
- Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
- Country of Origin United States
- Expiration 12 months from receipt of product.
- Shipping Ice packs
Customer reviews
No reviews were found for Aves - Model GFP-1020 - Anti-Green Fluorescent Protein Antibody. Be the first to review!