Flow CAST -Cellular Allergy Stimulation Test
The Flow CAST®(Cellular Allergy Stimulation Test) is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination – CCR3 and CD63 – combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one. Thanks to its easy protocol and smart marker combination the first results can be obtained within one hour – perfect conditions for laboratories.
CCR3 as Basophil Marker
CCR3 is detected with a PE-fluorescence labelled monoclonal antibody. This gating of basophil cells is significantly easier than with any other commercially available BAT.
CD63 as Activation Marker
The cell surface marker of basophils, CD63 is detected by a FITC-fluorescence labeled monoclonal antibody. CD63 is currently the best-validated basophil activation marker directly linked to basophil degranulation.
Controls
The first control, anti-FcEpsilonRI, is a highly specific monoclonal antibody binding to IgE receptor. The second control, a tripetide (fMLP) activates the basophils by a non-immunological way.
CD203c as Activation Marker
The CD203c is an other basophil activation marker in addition to the CD63. Our CD203c Reagent Set (RUO) is intended for the use within an extended protocol for the Flow CAST® kit, allowing the measurement of both activation markers, CD63 and CD203c, with two different fluorescence labelled antibodies.
Method: Flow Cytometry
Time to result: 1 h (approx.)
Sample type: 50 µl EDTA whole blood
Marker: CCR3-PE / CD63-FITC
Patents: US 8,563,258 B2; EP 2 037 269 B1
Status: RUO
Method: Flow Cytometry
Time to result: 1 h (approx.)
Sample type: 50 µl EDTA whole blood
Marker: CCR3-PE / CD63-FITC / CD203c-PE-DY647