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Aldevron - Model IVT - Enzymes
Our in vitro transcription (IVT) enzymes are qualified both by activity assays and functionally for RNA synthesis and can be custom manufactured to meet your specific project requirements from milligram to multigram scale. View the table below for a summary of our IVT enzymes.
Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
2’-O-Methyltransferase
2`-O-Methyltransferase adds a methyl group to the 2`-OH of the first nucleotide adjacent to the m7Gppp5`N cap. 2`-O-Methyltransferase utilizes
S-adenosylmethionine as the methyl group donor.