Epigenetic Antibodies

The histone H3 of certain tumors is methylated at the level of a lysine in an area which is highly conserved and identical in humans and mice. Volition company initially worked with a polyclonal but with the specificity problems that this entails. After several unsuccessful attempts to obtain a monoclonal antibody directed against this methylated lysine in mice and rabbits, Volition then entrusted this work to Synabs to try to obtain a monoclonal in rat species. Final obtained rat mAbs make the difference between methylated and non-methylated lysine on histone DNA. An immuno-assay has been consequently developed under NuQ brand and is now marketed.