ScienCell -Model 1000 -Human Brain Microvascular Endothelial Cells
Brain microvascular endothelial cells (BMEC), the major component of the blood-brain barrier, limit the passage of soluble and cellular substances from the blood into the brain. BMEC have unique features to distinguish themselves from those of peripheral endothelial cells, such as 1) intercellular tight junctions that display high electrical resistance and slow paracellular flux, 2) the absence of fenestrae and a reduced level of pinocytic activity, and 3) asymmetricallylocalized enzymes and carrier-mediated transport systems. Similar to peripheral endothelial cells, BMEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. BMEC have been widely used for studying the molecular and cellular properties of blood-brain barrier because of their unique functions.
- Catalog No.: 1000
- Country of Manufacture: United States
- Product Code: HBMEC
- Size/Quantity: 5 x 105 cells/vial.
- Product Use: HBMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
- Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
- Shipping Info: Dry ice.
- References:
- [1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55.
- [2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14.
- [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.
Isolated from human brain. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume.
HBMEC from ScienCell Research Laboratories are isolated from human brain. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HBMEC are characterized by immunofluorescence with antibodies specific to vWF and/or CD31 (PECAM1). HBMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HBMEC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HBMEC are not recommended for expanding or long-term cultures due to limited expansion capacity.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HBMEC in vitro.