Human Circulating Tumour Cells (CTC)
Human circulating tumour cells (CTC) in blood are an important indicator of the progression of a cancer and in some cases, these may be an indication of so called sleeping tumour cells i.e. it does mean that there is an active disease. Therefore PCR tests are being conducted in many laboratories around the world for presence of markers of such blood cells. As the number of such cell is very low, therefore there is a need of enrichment of these cells before doing RNA isolation.
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Genekam Biotechnology AG has developed this enrichment step and this can be combined with mini-column method or other method. Genekam offers this enrichment step as sterilised product because some users may want to grow enriched cells in order to do other studies including nucleic cells isolation e.g. tumour resistance (if possible!), but that one can go directly to RNA isolation step after enrichment even under unsterilised environment in order to run the tumour marker PCRassay. There is only 1 circulating tumour cell in one million of mononuclear cells, therefore enrichment is very important step during the conducting PCR for human cancer markers from blood. Peripheral blood is one of easiest way to access against the biopsy of lymphnodes or bone marrow, hence it is an excellent idea, if one can develop more and more assays based on CTC.
Important feature of enrichment step is that one can do the isolation of circulating tumour cells directly from blood 1ml or 3ml and there is no need of using density gradient method.
Genekam CTC Enrichment kit is very simple to use: There are only 3 steps:
- isolation of all cells from 1 or 2 ml of blood to develop a pellet
- adding of the beads to this pellet
- Isolation of specific cells - now you can use them for mini-column isolation or your in-house nucleic acid isolation
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