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Promega FcyRIIb - Model CHO-K1 - Immune Checkpoint Bioassays Cells
The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be cross-linked via binding to FcγRIIb receptors on CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., OX40, CD40, GITR and 4-1BB) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems. FcγRIIb CHO-K1 Cells are engineered to express human FcγRIIb, provided in cryopreserved thaw-and-use format. These are not reporter cells, and function solely to crosslink anti-CD40, anti-OX40, anti-4-1BB or anti-GITR antibodies.
Standardized Reagents Improve Results
- Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
- Kit includes all of the required reagents in standardized formats.
- Easily amenable to high-throughput workflows.
Representation of co-stimulatory bioassays (CD40, OX40, GITR or 4-1BB) with FcγRIIb-independent and FcγRIIb-dependent antibodies. Panel A. Assay with FcγRIIb-independent antibody. The bioassay consists of one engineered cell line: CD40, OX40, GITR or 4-1BB Effector Cells. In the absence of agonist antibody or ligand, the CD40, OX40, GITR or 4-1BB receptor is not activated and luminescence signal is low. The addition of agonist antibody or ligand induces the CD40, OX40, GITR or 4-1BB pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer. Panel B. Assay with FcγRIIb-dependent antibody. The bioassay consists of two engineered cell lines: CD40, OX40, GITR or 4-1BB Effector Cells and FcγRIIb CHO-K1 Cells. In the presence of FcγRIIb CHO-K1 Cells, the anti-CD40, -OX40, -GITR or 4-1BB antibody is cross-linked, thereby inducing CD40, OX40, GITR or 4-1BB pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer.
Product Kit Formats and Related Products- Kits are available in 1X and 5X sizes.
- Also available in CPM format (Cat.# J2232).
- 4-1BB, CD40, OX40 and GITR Bioassays are available separately.