InVivo - Large-Scale Production of Recombinant Antibodies And Proteins
Transient gene expression in mammalian cells provides reasonable amounts of recombinant proteins and antibodies which is sufficient for most applications. Nevertheless, when it comes to bulk productions for in vitro diagnostics or pre-clinical studies for biopharmaceutical development, the required amount of biological material often exceeds the yield that can be obtained through transient approaches. In this case, production from stable cell lines is highly advantageous since it allows easy generation of larger quantities of recombinant proteins and antibodies in measures of up to several grams per batch.
Details
However, developing a stable cell line can be a time and resource intensive process with several bottlenecks that need to be overcome in order to ensure a good yield output.
After years of optimization work to counteract these bottlenecks, InVivo successfully established a proprietary platform for the development of stable cell lines using CHO (Chinese hamster ovary) cells as expression system.
We chose a well-established, cGMP-banked CHO cell line and combined it with our optimized cell culture processes using a high-performance, chemically-defined, animal component-free medium.
Novel expression vectors compatible to the PiggyBac system – a well-established tool for transposon-mediated active gene transfer, were designed. Using these customized vectors, high protein yields and a substantially reduced timeline from transfection to stable CHO cell pools could be achieved.
To further increase product yield, high cell-density fermentation processes were established.
All these combined with a highly optimized process for the selection of best-producing cell pools and screening for high-producing monoclonals, lead to cost-optimized production of target proteins in large quantities.
We understand that every project is unique. Therefore, the entire process can be individually tailored to fit your specific requirements. After detailed rounds of discussion with our scientific experts, we help you decide if a stable cell line needs to be developed or if we can produce the required protein yields from a stable cell pool. With your final go, we start right away.
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