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Nippon Bambanker Direct - Model BBD01 -Serum-Free Cryopreservation Media for Long Storage of Hybridomas
Enables long term cell storage at -80°C or -196°C. Perfectly suited for hybridoma cells and HTP-applications. Bambanker Direct can be directly added to the cells – No washing and no centrifugation necessary. Improved cell recovery and viability after thawing. Ready-to-use freezing media – no programmed or sequential freezing required. Serum-free – no risk of contamination. GMP quality (more information). Long shelf life.
Bambanker™ Direct for hybridomas
There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that Bambanker™ Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add Bambanker™ Direct, one-toone, with your cells in media and directly place in freezer. And like with the original Bambanker™, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.
Procedure for the cryopreservation of cells

GMP quality
All products of the Bambanker™ series are manufactured in facilities compliant with current good manufacturing practices (GMP).
The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
Notification: Manufacturer’s notification for Bambanker as GMP-manufactured product (pdf file)
Flexible
There a four different versions of Bambanker™.
- All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
- They also do not contain antibiotic or antifungal ingredients.
The four different types of Bambanker™ are recommended depending on your specific needs:
Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 100 mL)
Please note that the 120 mL bottle size (BB01) is no longer available and has been replaced by the new 100 mL bottle size (BB05).
Bambanker™ Direct (for hybridoma cells, package unit: 20 mL)
Bambanker™ DMSO Free (for high sensitivity cells, package unit: 20 mL)
Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 100 mL)
Please note that the 120 mL bottle size (BBH03) is no longer available and has been replaced by the new 100 mL bottle size (BBH04)
- C2C12 (mouse skeletal muscle cell line)
- Daudi (human B cell line)
- HEK293; HEK293T (human embryonic kidney cell line)
- HeLa/HeLa S3 (human cervical cancer cell line)
- HepG2 (human liver cancer cell line)
- Jurkat (human leukemia T cell line)
- K562 (human chronic myelogenous leukemia cell line)
- KATO (human gastric epithelial cell line)
- MDCK (canine kidney tubular epithelial cell line)
- NIH3T3 (mouse embryonic skin cell line)
- OKT4 (mouse hybridoma)
- OP9 (mouse myeloid stromal cells)
- PC12 (rat adrenal medulla cell line)
- SNL (mouse embryonic fibroblasts)
- ES (mouse embryonic stem cells)
- MEF (mouse embryonic fibroblasts)
- Vero (African green monkey kidney cell line)
- OP9 (mouse myeloma stroma cells)
- P3U1 (mouse myeloma cell line)
and many more…
