Nippon Bambanker DMSO Free - Model BBF01 -Serum-Free Cryopreservation Media for Most Sensitive Cells
DMSO-free – for the most sensitive cells. Enables long term cell storage at -80°C or -196°C. Improved cell recovery and viability after thawing. Ready-to-use freezing media – no programmed or sequential freezing required. Serum-free – no risk of contamination. GMP manufactured. Usable for all known cell lines. Long shelf life.
No more DMSO – for the most sensitive cells
DMSO is added to most freezing reagents to help avoid formation of ice crystals, which of course harm the cells. However, DMSO is cytotoxic and can reduce the survival rate of certain sensitive cell lines. Bambanker DMSO Free is made without DMSO. Instead, it uses a unique formulation to avoid the formation of ice crystals. This makes Bambanker DMSO Free especially suitable for cell lines that proved to be sensitive to DMSO under long-term storage conditions.
Flexible
There a four different versions of Bambanker™.
- All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
- They also do not contain antibiotic or antifungal ingredients.
The four different types of Bambanker™ are recommended depending on your specific needs:
Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 100 mL)
Please note that the 120 mL bottle size (BB01) is no longer available and has been replaced by the new 100 mL bottle size (BB05).
Bambanker™ Direct (for hybridoma cells, package unit: 20 mL)
Bambanker™ DMSO Free (for high sensitivity cells, package unit: 20 mL)
Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 100 mL)
Please note that the 120 mL bottle size (BBH03) is no longer available and has been replaced by the new 100 mL bottle size (BBH04).
All products of the Bambanker series are manufactured in facilities compliant with current good manufacturing practices (GMP).
The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
NotificationManufacturer’s notification for Bambanker as GMP-manufactured product (pdf file)
The following cell lines have successfully been frozen with Bambanker DMSO-free:
- Jurkat (Floating cells)
- PC12 (Adrenal Pheochromocytoma, Rat) (Adhesive cells)
- HepG2 (Adhesive cells)
- Hela S3 (Epithelioid Carcinoma, Cervix, Human)(Adhesive cells)
- HEK293T (Transformed Primary Embryonal Kidney, Human)(Adhesive cells)
- OKT4 (Floating cells)
- Vero P4 (Kidney, African Green Monkey(Adhesive cells)
- MDCK (Kidney, Canine)(Adhesive cells)
- NIH3T3 (Embryonic Fibroblast, Mouse)(Adhesive cells)
I consider Bambanker a very useful worktool for the following reasons:
1) replacement of a toxic substance (DMSO)
2) saving of 4 hours of time required for freezing in nitrogen / -80° C foreseen with the use of DMSO
3) cellular vitality guaranteed after the defrosting of all the linesthat I manage
Dott.ssa Allessandra Leone, Department of Virology and Cell Culture, IZSAM “G.Caporale” Teramo
Literature referenceEffect of DMSO on cell differentitation:
Jiang, C. et al. Down-regulation of TRRAP-dependent hTERT and TRRAP-independent CAD activation by Myc/Max contributes to the differentiation of HL60 cells after exposure to DMSO International Immunopharmacology, 6 (7), 1204-1213 (2006)