Aldevron -Model pALD-AAV Rep/Cap -Plasmids
Recombinant AAV (rAAV) manufacturing is composed of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.
Aldevron`s pALD-AAV rep/cap plasmids have several advantages:
- Royalty free, including for commercial products
- Kanamycin resistance
- Consistent, reliable supply
- Immediately available at research grade
With Aldevron`s rep/cap plasmids, clients do not need to manufacture a custom batch as Aldevron maintains each as an in-stock product, reducing timeline and cost.
Each rep/cap plasmid contains the gene sequence for AAV serotype 2 replication (rep) protein paired with either serotype 2, 5 or 6 capsid (cap) protein respectively. Use of AAV serotype 2 rep proteins in combination with other serotypes for cap proteins is referred to as pseudotyping which provides enhanced and refined tissue tropism in some applications.